BMRB Entry 4854

Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4854

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Title:
Chemical shift assignments for the isolated N-terminal domain of EPSP synthase
Deposition date:
2000-10-12
Original release date:
2001-11-14
Authors:
Stauffer, Melissa; Young, John; Helms, Greg; Evans, Jeremy
Citation:

Citation: Stauffer, Melissa; Young, John; Helms, Greg; Evans, Jeremy. "Letter to the Editor: Sequential Assignments of the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase"  J. Biomol. NMR 20, 387-388 (2001).
PubMed: 11563562

Assembly members:

Assembly members:
N-terminal EPSP synthase, polymer, 240 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
N-terminal EPSP synthase: MESLTLQPIARVDGTINLPG SKSVSNRALLLAALAHGKTV LTNLLDSDDVRHMLNALTAL GVSYTLSADRTRCEIIGNGG PLHAEGALELFLGNAGTAMR PLAAALCLGSNDIVLTGEPR MKERPIGHLVDALRLGGAKI TYLEQENYPPLRLQGGFTGG NVDVDGSVSSQFLTALLMTA PLAPEDTVIRIKGDLVSKPY IDITLNLMKTFGVEIENQHY QQFVVKGGQSYQSPGTYLVE

Data sets:
Data typeCount
1H chemical shifts1410
13C chemical shifts867
15N chemical shifts195

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain EPSPs1

Entities:

Entity 1, N-terminal domain EPSPs 240 residues - Formula weight is not available

1   METGLUSERLEUTHRLEUGLNPROILEALA
2   ARGVALASPGLYTHRILEASNLEUPROGLY
3   SERLYSSERVALSERASNARGALALEULEU
4   LEUALAALALEUALAHISGLYLYSTHRVAL
5   LEUTHRASNLEULEUASPSERASPASPVAL
6   ARGHISMETLEUASNALALEUTHRALALEU
7   GLYVALSERTYRTHRLEUSERALAASPARG
8   THRARGCYSGLUILEILEGLYASNGLYGLY
9   PROLEUHISALAGLUGLYALALEUGLULEU
10   PHELEUGLYASNALAGLYTHRALAMETARG
11   PROLEUALAALAALALEUCYSLEUGLYSER
12   ASNASPILEVALLEUTHRGLYGLUPROARG
13   METLYSGLUARGPROILEGLYHISLEUVAL
14   ASPALALEUARGLEUGLYGLYALALYSILE
15   THRTYRLEUGLUGLNGLUASNTYRPROPRO
16   LEUARGLEUGLNGLYGLYPHETHRGLYGLY
17   ASNVALASPVALASPGLYSERVALSERSER
18   GLNPHELEUTHRALALEULEUMETTHRALA
19   PROLEUALAPROGLUASPTHRVALILEARG
20   ILELYSGLYASPLEUVALSERLYSPROTYR
21   ILEASPILETHRLEUASNLEUMETLYSTHR
22   PHEGLYVALGLUILEGLUASNGLNHISTYR
23   GLNGLNPHEVALVALLYSGLYGLYGLNSER
24   TYRGLNSERPROGLYTHRTYRLEUVALGLU

Samples:

sample_1: N-terminal EPSP synthase, [U-15N; U-13C; U-70% 2H], 1.0 mM; Tris 50 mM; DTT 1 mM

conditions_1: pH*: 7.8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions
15N-HSQCnot availablenot availablenot available
HNCAnot availablenot availablenot available
HN(CO)CAnot availablenot availablenot available
HNCACBnot availablenot availablenot available
HNCO,not availablenot availablenot available
HN(CA)COnot availablenot availablenot available
1H-15N NOESY-HSQCnot availablenot availablenot available

Software:

NMRPipe - data processing

PIPP - peak picking for assignments

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 4848
PDB
DBJ BAA35643 BAB34414 BAG76491 BAI24350 BAI29802
EMBL CAA25223 CAP75378 CAQ31436 CAQ97812 CAR02268
GB AAC32745 AAC72854 AAC73994 AAG55393 AAN42533
PRF 1006223A
REF NP_286783 NP_309018 NP_415428 NP_706826 NP_752973
SP A1A9I5 A7ZJZ7 A7ZYL1 B1IW23 B1LJV6
AlphaFold A1A9I5 A7ZJZ7 A7ZYL1 B1IW23 B1LJV6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks