BMRB Entry 4848

Title:
Conformational Changes in the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase upon Shikimate-3-phosphate Binding
Deposition date:
2000-10-04
Original release date:
2001-11-14
Authors:
Stauffer, Melissa; Young, John; Helms, Greg; Evans, Jeremy
Citation:

Citation: Stauffer, Melissa; Young, John; Helms, Greg; Evans, Jeremy. "Letter to the Editor: Sequential Assignments of the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase"  J. Biomol. NMR 20, 387-388 (2001).
PubMed: 11563562

Assembly members:

Assembly members:
EPSP synthase, polymer, 240 residues, Formula weight is not available
shikimate-3-phosphate, non-polymer, 254.131 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Eschericia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Data sets:
Data typeCount
1H chemical shifts1546
13C chemical shifts906
15N chemical shifts218

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1N-terminal domain1
2shikimate-3-phosphate2

Entities:

Entity 1, N-terminal domain 240 residues - Formula weight is not available

1   METGLUSERLEUTHRLEUGLNPROILEALA
2   ARGVALASPGLYTHRILEASNLEUPROGLY
3   SERLYSSERVALSERASNARGALALEULEU
4   LEUALAALALEUALAHISGLYLYSTHRVAL
5   LEUTHRASNLEULEUASPSERASPASPVAL
6   ARGHISMETLEUASNALALEUTHRALALEU
7   GLYVALSERTYRTHRLEUSERALAASPARG
8   THRARGCYSGLUILEILEGLYASNGLYGLY
9   PROLEUHISALAGLUGLYALALEUGLULEU
10   PHELEUGLYASNALAGLYTHRALAMETARG
11   PROLEUALAALAALALEUCYSLEUGLYSER
12   ASNASPILEVALLEUTHRGLYGLUPROARG
13   METLYSGLUARGPROILEGLYHISLEUVAL
14   ASPALALEUARGLEUGLYGLYALALYSILE
15   THRTYRLEUGLUGLNGLUASNTYRPROPRO
16   LEUARGLEUGLNGLYGLYPHETHRGLYGLY
17   ASNVALASPVALASPGLYSERVALSERSER
18   GLNPHELEUTHRALALEULEUMETTHRALA
19   PROLEUALAPROGLUASPTHRVALILEARG
20   ILELYSGLYASPLEUVALSERLYSPROTYR
21   ILEASPILETHRLEUASNLEUMETLYSTHR
22   PHEGLYVALGLUILEGLUASNGLNHISTYR
23   GLNGLNPHEVALVALLYSGLYGLYGLNSER
24   TYRGLNSERPROGLYTHRTYRLEUVALGLU

Entity 2, shikimate-3-phosphate 1 residues - C7 H11 O8 P - 254.131 Da.

1   S3P

Samples:

Nterm+S3P: EPSP synthase, [U-15N; U-13C; U70%-2H], 1.0 mM; shikimate-3-phosphate 1.5 mM; Tris buffer 50 mM; DTT 1 mM; NaN3 3 mM; pepstatin uM; leupeptin uM

Ex-cond_1: pH*: 7.8; temperature: 295 K

Experiments:

NameSampleSample stateSample conditions

Software:

No software information available

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 4854
PDB
DBJ BAA35643 BAB34414 BAG76491 BAI24350 BAI29802
EMBL CAA25223 CAP75378 CAQ31436 CAQ97812 CAR02268
GB AAC32745 AAC72854 AAC73994 AAG55393 AAN42533
PRF 1006223A
REF NP_286783 NP_309018 NP_415428 NP_706826 NP_752973
SP A1A9I5 A7ZJZ7 A7ZYL1 B1IW23 B1LJV6
AlphaFold A1A9I5 A7ZJZ7 A7ZYL1 B1IW23 B1LJV6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks