BMRB Entry 4811

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4811

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H and 15N Chemical Shift Assignments for Apo-Recombinant Bovine alpha-Lactalbumin

Deposition date:

2000-08-17

Original release date:

2000-11-13

Authors:

Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina

Citation:

Citation: Wijesinha-Bettoni, Ramani; Dobson, Christopher; Redfield, Christina. "Comparison of the Structural and Dynamical Properties of Holo and Apo bovine Alpha-lactalbumin by NMR Spectroscopy" J. Mol. Biol. 307, 885-898 (2001).
PubMed: 11273708

Assembly members:

Assembly members:
apo bovine alpha-lactalbumin, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: bovine Taxonomy ID: 9909 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Bos Primigenius

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet3a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
apo bovine alpha-lactalbumin: MEQLTKCEVFRELKDLKGYG GVSLPEWVCTTFHTSGYDTE AIVQNNDSTEYGLFQINNKI WCKNDQDPHSSNICNISCDK FKDDDLTDDIVCVKKILDKV GINYWLAHKALCSEKLDQWL CEKL

Data sets:

assigned_chemical_shifts
- apo-bla_backbone1

Data type	Count
15N chemical shifts	115
1H chemical shifts	217

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	apo-BLA	1

Entities:

Entity 1, apo-BLA 124 residues - Formula weight is not available

1

MET

GLU

GLN

LEU

THR

LYS

CYS

GLU

VAL

PHE

2

ARG

GLU

LEU

LYS

ASP

LEU

LYS

GLY

TYR

GLY

3

GLY

VAL

SER

LEU

PRO

GLU

TRP

VAL

CYS

THR

4

THR

PHE

HIS

THR

SER

GLY

TYR

ASP

THR

GLU

5

ALA

ILE

VAL

GLN

ASN

ASP

SER

THR

GLU

6

TYR

GLY

LEU

PHE

GLN

ILE

ASN

LYS

ILE

7

TRP

CYS

LYS

ASN

ASP

GLN

ASP

PRO

HIS

SER

8

SER

ASN

ILE

CYS

ASN

ILE

SER

CYS

ASP

LYS

9

PHE

LYS

ASP

LEU

THR

ASP

ILE

10

VAL

CYS

VAL

LYS

ILE

LEU

ASP

LYS

VAL

11

GLY

ILE

ASN

TYR

TRP

LEU

ALA

HIS

LYS

ALA

12

LEU

CYS

SER

GLU

LYS

LEU

ASP

GLN

TRP

LEU

13

CYS

GLU

LYS

LEU

Samples:

apo-bla_15N_sample: apo bovine alpha-lactalbumin, [U-15N], 2.0 mM; EDTA 15 mM; NaCl 0.5 M

Ca2+_free_conditions: ionic strength: 0.5 M; pH: 7.1; temperature: 293 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	apo-bla_15N_sample	not available	Ca2+_free_conditions
3D 1H-15N NOESY-HSQC	apo-bla_15N_sample	not available	Ca2+_free_conditions
2D 1H pre-TOCSY COSY	apo-bla_15N_sample	not available	Ca2+_free_conditions

Software:

FELIX v2.30 - data processing

NMR spectrometers:

Home-built . 750 MHz