BMRB Entry 4332

Title:
1H and 15N Chemical Shift Assignments for Recombinant Bovine Alpha-Lactalbumin
Deposition date:
1999-04-12
Original release date:
1999-05-25
Authors:
Forge, Vincent; Wijesinha, Ramani; Balbach, Jochen; Brew, Keith; Robinson, Carol; Redfield, Christina; Dobson, Christopher
Citation:

Citation: Forge, Vincent; Wijesinha, Ramani; Balbach, Jochen; Brew, Keith; Robinson, Carol; Redfield, Christina; Dobson, Christopher. "Rapid Collapse and Slow Structural Reorganization During the Refolding of Bovine Alpha-Lactalbumin"  J. Mol. Biol. ., .-. (1999).

Assembly members:

Assembly members:
bovine alpha-lactalbumin, polymer, 124 residues, Formula weight is not available
CALCIUM ION, non-polymer, 40.078 Da.
HOH, non-polymer, 18.015 Da.

Natural source:

Natural source:   Common Name: bovine   Taxonomy ID: 9913   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Bos taurus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet3a

Data sets:
Data typeCount
15N chemical shifts118
1H chemical shifts232

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1bla monomer1
2CA22
3HOH3
4HoH3

Entities:

Entity 1, bla monomer 124 residues - Formula weight is not available

1   METGLUGLNLEUTHRLYSCYSGLUVALPHE
2   ARGGLULEULYSASPLEULYSGLYTYRGLY
3   GLYVALSERLEUPROGLUTRPVALCYSTHR
4   THRPHEHISTHRSERGLYTYRASPTHRGLU
5   ALAILEVALGLNASNASNASPSERTHRGLU
6   TYRGLYLEUPHEGLNILEASNASNLYSILE
7   TRPCYSLYSASNASPGLNASPPROHISSER
8   SERASNILECYSASNILESERCYSASPLYS
9   PHELEUASPASPASPLEUTHRASPASPILE
10   VALCYSVALLYSLYSILELEUASPLYSVAL
11   GLYILEASNTYRTRPLEUALAHISLYSALA
12   LEUCYSSERGLULYSLEUASPGLNTRPLEU
13   CYSGLULYSLEU

Entity 2, CA2 - Ca - 40.078 Da.

1   CA

Entity 3, HOH - H2 O - 18.015 Da.

1   HOH

Samples:

bla_15N_sample: bovine alpha-lactalbumin, [U-15N], 2.0 mM; CaCl2 5 mM; D2O 5%; H2O 95%

sample_conditions: pH: 6.3 na; temperature: 293 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCbla_15N_samplenot availablesample_conditions
3D 1H-15N TOCSY-HSQCbla_15N_samplenot availablesample_conditions
3D 1H-15N NOESY-HSQCbla_15N_samplenot availablesample_conditions
2D 1H pre-TOCSY COSYbla_15N_samplenot availablesample_conditions

Software:

FELIX v2.30 - data processing

NMR spectrometers:

  • Home-built . 750 MHz

Related Database Links:

PDB
PIR LABOZ

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks