BMRB Entry 4767

Title:
1Ha and 13Ca, 13Cb shifts of apocytochrome b562 at pH 5.1 (temperature 293 K)
Deposition date:
2000-06-20
Original release date:
2002-09-23
Authors:
D'Amelio, Nicola; Bonvin, Alexandre; Czisch, Michael; Barker, Paul; Kaptein, Rob
Citation:

Citation: D'Amelio, Nicola; Bonvin, Alexandre; Czisch, Michael; Barker, Paul; Kaptein, Rob. "The C terminus of apocytochrome b562 undergoes fast motions and slow exchange among ordered conformations resembling the folded state"  Biochemistry 41, 5505-5514 (2002).
PubMed: 11969411

Assembly members:

Assembly members:
apocytochrome b562, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts108
13C chemical shifts303

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo b5621

Entities:

Entity 1, apo b562 106 residues - Formula weight is not available

1   ALAASPLEUGLUASPASNMETGLUTHRLEU
2   ASNASPASNLEULYSVALILEGLULYSALA
3   ASPASNALAALAGLNVALLYSASPALALEU
4   THRLYSMETARGALAALAALALEUASPALA
5   GLNLYSALATHRPROPROLYSLEUGLUASP
6   LYSSERPROASPSERPROGLUMETLYSASP
7   PHEARGHISGLYPHEASPILELEUVALGLY
8   GLNILEASPASPALALEULYSLEUALAASN
9   GLUGLYLYSVALLYSGLUALAGLNALAALA
10   ALAGLUGLNLEULYSTHRTHRARGASNALA
11   TYRHISGLNLYSTYRARG

Samples:

sample_1: apocytochrome b562, [U-15N; U-13C], 1 mM

condition_1: pH: 5.1; temperature: 293 K; ionic strength: 0.2 M

Experiments:

NameSampleSample stateSample conditions
HNCOnot availablenot availablenot available
HBHA(CBCACO)NHnot availablenot availablenot available
HNCACBnot availablenot availablenot available
CBCA(CO)NHnot availablenot availablenot available
1H-15N TOCSY-HSQCnot availablenot availablenot available

Software:

REGINE -

NMR spectrometers:

  • Varian Inova 500 MHz

Related Database Links:

BMRB 1672 4759
PDB
DBJ BAB38636 BAG80065 BAI28542 BAI33713 BAI38842
EMBL CAA47706 CAP78754 CAQ34585 CAQ91733 CAR01210
GB AAA97133 AAB20782 AAG59433 AAN45672 AAN83756
PIR E86121 E91280
REF NP_290867 NP_313240 NP_709965 NP_757182 WP_000112424
SP P0ABE7 P0ABE8 Q8CVG7
AlphaFold P0ABE7 P0ABE8 Q8CVG7