BMRB Entry 4763

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4763

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

15N relaxation data and model-free parameters for the N-terminal receiver domain of double-mutant (D86N/A89T) unphosphorylated NtrC receiver domain (NtrCr(D86N/A89T))

Deposition date:

2000-06-14

Original release date:

2002-04-11

Authors:

Kern, D.; Volkman, B.; Wemmer, D.

Citation:

Citation: Volkman, Brian; Lipson, Doron; Wemmer, David; Kern, Dorothee. "Two-state allosteric behavior in a single domain signaling protein" Science 291, 2429-2433 (2001).

Assembly members:

Assembly members:
Nitrogen regulatory protein C N-terminal receiver domain, polymer, 124 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Bacteria Taxonomy ID: 602 Superkingdom: Eubacteria Kingdom: not available Genus/species: Salmonella typhimurium

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Nitrogen regulatory protein C N-terminal receiver domain: MQRGIVWVVDDDSSIRWVLE RALAGAGLTCTTFENGNEVL AALASKTPDVLLSDIRMPGM DGLALLKQIKQRHPMLPVII MTAHSNLDTAVSAYQQGAFD YLPKPFDIDEAVALVERAIS HYQE

Data sets:

heteronucl_NOEs
- heteronuclear_NOE_750
heteronucl_T1_relaxation
- T1_set_750
heteronucl_T2_relaxation
- T2_relaxation_750
- T2_relaxation_600

Data type	Count
T1 relaxation values	97
T2 relaxation values	194

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	NITROGEN REGULATION PROTEIN	1

Entities:

Entity 1, NITROGEN REGULATION PROTEIN 124 residues - Formula weight is not available

1

MET

GLN

ARG

GLY

ILE

VAL

TRP

VAL

ASP

2

ASP

SER

ILE

ARG

TRP

VAL

LEU

GLU

3

ARG

ALA

LEU

ALA

GLY

ALA

GLY

LEU

THR

CYS

4

THR

PHE

GLU

ASN

GLY

ASN

GLU

VAL

LEU

5

ALA

LEU

ALA

SER

LYS

THR

PRO

ASP

VAL

6

LEU

SER

ASP

ILE

ARG

MET

PRO

GLY

MET

7

ASP

GLY

LEU

ALA

LEU

LYS

GLN

ILE

LYS

8

GLN

ARG

HIS

PRO

MET

LEU

PRO

VAL

ILE

9

MET

THR

ALA

HIS

SER

ASN

LEU

ASP

THR

ALA

10

VAL

SER

ALA

TYR

GLN

GLY

ALA

PHE

ASP

11

TYR

LEU

PRO

LYS

PRO

PHE

ASP

ILE

ASP

GLU

12

ALA

VAL

ALA

LEU

VAL

GLU

ARG

ALA

ILE

SER

13

HIS

TYR

GLN

GLU

Samples:

sample_1: Nitrogen regulatory protein C N-terminal receiver domain, [U-99% 15N], 0.9 mM; phosphate buffer 50 mM

sample_cond_1: ionic strength: 50 mM; pH: 6.75; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	not available	sample_cond_1

Software:

FELIX v95.0 - processing

xwinnmr v2.1 - collection

ModelFree v4.01 - data analysis

NMR spectrometers:

BRUKER DMX 600 MHz
BRUKER DMX 750 MHz

BMRB	4527 4528 4762 4764 5303
PDB	1DC7 1DC8 1J56 1KRW 1KRX 1NTR
DBJ	BAJ39092 BAP09964
EMBL	CAA59425 CAD03095 CAR35368 CAR39210 CAR61856
GB	AAL22844 AAO71117 AAV79614 AAX67803 ABX70260
PIR	AC0950
REF	NP_458044 NP_462885 NP_807257 WP_001188773 WP_001188774
SP	P41789
AlphaFold	P41789