BMRB Entry 4702

Title:
Structure of the Negative Regulatory Domain of p53 bound to S100B
Deposition date:
2000-03-27
Original release date:
2000-12-18
Authors:
Rustandi, Richard; Baldisseri, Donna; Weber, David
Citation:

Citation: Rustandi, Richard; Baldisseri, Donna; Weber, David. "Structure of the Negative Regulatory Domain of p53 bound to S100B"  Nat. Struct. Biol. 7, 570-574 (2000).

Assembly members:

Assembly members:
S100B beta, polymer, 91 residues, Formula weight is not available
C-terminal p53, polymer, 24 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus novergicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Data sets:
Data typeCount
1H chemical shifts113

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1a chain1
2b chain1
3Ca 13
4Ca 23
5C-terminal p53 12
6C-terminal p53 22

Entities:

Entity 1, a chain 91 residues - Formula weight is not available

1   SERGLULEUGLULYSALAMETVALALALEU
2   ILEASPVALPHEHISGLNTYRSERGLYARG
3   GLUGLYASPLYSHISLYSLEULYSLYSSER
4   GLULEULYSGLULEUILEASNASNGLULEU
5   SERHISPHELEUGLUGLUILELYSGLUGLN
6   GLUVALVALASPLYSVALMETGLUTHRLEU
7   ASPGLUASPGLYASPGLYGLUCYSASPPHE
8   GLNGLUPHEMETALAPHEVALSERMETVAL
9   THRTHRALACYSHISGLUPHEPHEGLUHIS
10   GLU

Entity 3, Ca 1 - Ca - 40.078 Da.

1   CA

Entity 2, C-terminal p53 1 24 residues - Formula weight is not available

1   ACESERHISLEULYSSERLYSLYSGLYGLN
2   SERTHRSERARGHISLYSLYSLEUMETPHE
3   LYSTHRGLUNH2

Samples:

sample_1: C-terminal p533 – 7 mM

condition_1: pH: 6.5; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 12C filter NOESY and TOCSYsample_1not availablecondition_1
2D 12C, 14N filter NOESY and TOCSYsample_1not availablecondition_1
2D NOESY and 2D TOCSYsample_1not availablecondition_1
2D 14N filter NOESY and TOCSYsample_1not availablecondition_1

Software:

NMRPipe v2.0 - Process and analyse the NMR data

NMR spectrometers:

  • Bruker DMX 600 MHz

Related Database Links:

BMRB 15923 4001 4099 4105 4099
PDB
DBJ BAB43945 BAE22214 BAE22413 BAE36647 BAE88979 BAC16799.1 BAD96746.1
EMBL CAA25567 CAG46920 CAA42635.1 CAA42627.1 CAA38095.1 CAA26306.1 CAJ28922.1
GB AAA03075 AAA42096 AAA60367 AAH01766 AAH61178
PRF 2003367B
REF NP_001076199 NP_001233735 NP_001247455 NP_001270589 NP_006263 XP_511957.2 NP_000537.3 XP_001172077.1 XP_001172061.1 XP_001172050.1
SP P04271 P04631 P50114 Q6YNR6
SWISS-PROT P04637
GenBank EAW90142.1 EAW90140.1 ABM86630.1 ABL09846.1 EAW90141.1
AlphaFold P04271 P04631 P50114 Q6YNR6