BMRB Entry 4001

Title:
1H, 13C, and 15N resonance assignments for reduced apo-S100beta
Deposition date:
1995-06-15
Original release date:
.
Authors:
Amburgey, Judith; Abildgaard, Frits; Starich, Mary; Shah, Sanjiv; Hilt, Dana; Weber, David
Citation:

Citation: Amburgey, Judith; Abildgaard, Frits; Starich, Mary; Shah, Sanjiv; Hilt, Dana; Weber, David. "1H, 13C and 15N NMR Assignments and Solution Secondary Structure of Rat Apo-S100beta"  J. Biomol. NMR 6, 171-179 (1995).

Assembly members:

Assembly members:
S100beta, polymer, 92 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11b containing the S100beta gene

Experimental source:

Natural source:   Common Name: rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET11b containing the S100beta gene

Entity Sequences (FASTA):

Data typeCount
13C chemical shifts452
15N chemical shifts114
1H chemical shifts711

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1apo-S100beta1

Entities:

Entity 1, apo-S100beta 92 residues - Formula weight is not available

1   METSERGLULEUGLULYSALAMETVALALA
2   LEUILEASPVALPHEHISGLNTYRSERGLY
3   ARGGLUGLYASPLYSHISLYSLEULYSLYS
4   SERGLULEULYSGLULEUILEASNASNGLU
5   LEUSERHISPHELEUGLUGLUILELYSGLU
6   GLNGLUVALVALASPLYSVALMETGLUTHR
7   LEUASPGLUASPGLYASPGLYGLUCYSASP
8   PHEGLNGLUPHEMETALAPHEVALSERMET
9   VALTHRTHRALACYSHISGLUPHEPHEGLU
10   HISGLU

Samples:

sample_one: S100beta3 – 6 mM; EDTA0.34 – 0.34 mM; beta-mercaptoethanol2 – 10 mM; d11-Tris-HCl6 – 10 mM; NaCl17 – 20 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.5 na; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

    Related Database Links:

    BMRB 15923 4099 4105 4702
    PDB
    DBJ BAB43945 BAE22214 BAE22413 BAE36647 BAE88979
    EMBL CAA25567 CAG46920
    GB AAA03075 AAA42096 AAA60367 AAH01766 AAH61178
    PRF 2003367B
    REF NP_001076199 NP_001233735 NP_001247455 NP_001270589 NP_006263
    SP P04271 P04631 P50114 Q6YNR6
    AlphaFold P50114

    Download HSQC peak lists in one of the following formats:
    CSV: Backbone or all simulated peaks
    SPARKY: Backbone or all simulated peaks