BMRB Entry 4700

Name: Structure of Cdc42 bound to the GTPase Binding Domain of PAK
Published: 2000-12-18

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PDB ID: 1cf4
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4700
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Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Structure of Cdc42 bound to the GTPase Binding Domain of PAK

Deposition date:

2000-03-23

Original release date:

2000-12-18

Authors:

Morreale, Angela; Venkatesan, Meenakshi; Mott, Helen; Owen, Darerca; Nietlispach, Daniel; Lowe, Peter; Laue, Ernest

Citation:

Citation: Morreale, Angela; Venkatesan, Meenakshi; Mott, Helen; Owen, Darerca; Nietlispach, Daniel; Lowe, Peter; Laue, Ernest. "Structure of Cdc42 bound to the GTPase Binding Domain of PAK" Nat. Struct. Biol. 7, 384-388 (2000).

Assembly members:

Assembly members:
Cdc42, polymer, 185 residues, 20430.40 Da.
p21 activated kinase, polymer, 46 residues, 5127.64 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cdc42: MQTIKCVVVGDGAVGKTCLL ISYTTNKFPSEYVPTVFDNY AVTVMIGGEPYTLGLFDTAG LEDYDRLRPLSYPQTDVFLV CFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLR DDPSTIEKLAKNKQKPITPE TAEKLARDLKAVKYVECSAL TQKGLKNVFDEAILAALEPP EPKKX
p21 activated kinase: GSISLPSDFEHTIHVGFDAV TGEFTGMPEQWARLLQTSNI TKSEQK

Data sets:

assigned_chemical_shifts
- shift_set_1
- shift_set_2

Data type	Count
13C chemical shifts	741
1H chemical shifts	1550
15N chemical shifts	224

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cdc42	1
2	PAK	2

Entities:

Entity 1, Cdc42 185 residues - 20430.40 Da.

1

MET

GLN

THR

ILE

LYS

CYS

VAL

GLY

2

ASP

GLY

ALA

VAL

GLY

LYS

THR

CYS

LEU

3

ILE

SER

TYR

THR

ASN

LYS

PHE

PRO

SER

4

GLU

TYR

VAL

PRO

THR

VAL

PHE

ASP

ASN

TYR

5

ALA

VAL

THR

VAL

MET

ILE

GLY

GLU

PRO

6

TYR

THR

LEU

GLY

LEU

PHE

ASP

THR

ALA

GLY

7

LEU

GLU

ASP

TYR

ASP

ARG

LEU

ARG

PRO

LEU

8

SER

TYR

PRO

GLN

THR

ASP

VAL

PHE

LEU

VAL

9

CYS

PHE

SER

VAL

SER

PRO

SER

PHE

10

GLU

ASN

VAL

LYS

GLU

LYS

TRP

VAL

PRO

GLU

11

ILE

THR

HIS

CYS

PRO

LYS

THR

PRO

PHE

12

LEU

VAL

GLY

THR

GLN

ILE

ASP

LEU

ARG

13

ASP

PRO

SER

THR

ILE

GLU

LYS

LEU

ALA

14

LYS

ASN

LYS

GLN

LYS

PRO

ILE

THR

PRO

GLU

15

THR

ALA

GLU

LYS

LEU

ALA

ARG

ASP

LEU

LYS

16

ALA

VAL

LYS

TYR

VAL

GLU

CYS

SER

ALA

LEU

17

THR

GLN

LYS

GLY

LEU

LYS

ASN

VAL

PHE

ASP

18

GLU

ALA

ILE

LEU

ALA

LEU

GLU

PRO

19

GLU

PRO

LYS

GNP

Entity 2, PAK 46 residues - 5127.64 Da.

1

GLY

SER

ILE

SER

LEU

PRO

SER

ASP

PHE

GLU

2

HIS

THR

ILE

HIS

VAL

GLY

PHE

ASP

ALA

VAL

3

THR

GLY

GLU

PHE

THR

GLY

MET

PRO

GLU

GLN

4

TRP

ALA

ARG

LEU

GLN

THR

SER

ASN

ILE

5

THR

LYS

SER

GLU

GLN

LYS

Samples:

sample_1: Cdc42, [U-13C; U-15N], 0.7 – 1.0 mM; p21 activated kinase, [U-13C; U-15N], 0.7 – 1.0 mM

Ex-cond_1: pH: 5.5; temperature: 298 K; ionic strength: 55 mM

Experiments:

Name	Sample	Sample state	Sample conditions
15N-edited NOESy	sample_1	not available	Ex-cond_1
13C-edited NOEsy	sample_1	not available	Ex-cond_1
HCCH-COSY	sample_1	not available	Ex-cond_1
HCCH-TOCSY	sample_1	not available	Ex-cond_1
HNCA	sample_1	not available	Ex-cond_1
HN(CO)CA	sample_1	not available	Ex-cond_1
CBCA(CO)NH	sample_1	not available	Ex-cond_1
HBHA(CBCACO)NH	sample_1	not available	Ex-cond_1
13C/15N-Xfiltered NOESY	sample_1	not available	Ex-cond_1

Software:

AZARA v3.3 - DATA PROCESSING

ANSIG - ASSIGNMENT

NMR spectrometers:

Bruker AMX 600 MHz
Bruker DRX 600 MHz
Bruker DRX 500 MHz
Bruker DRX 800 MHz

BMRB	15424
PDB	1A4R 1AJE 1AM4 1AN0 1CEE 1CF4 1DOA 1E0A 1EES 1GRN 1GZS 1KI1 1KZ7 1KZG 1NF3 2ASE 2DFK 2KB0 2NGR 2ODB 2QRZ 2WM9 2WMN 2WMO 3EG5 3GCG 3QBV 3VHL 4DID 4ITR 4JS0 4YC7 4YDH 1F3M 1E0A 2QME
DBJ	BAB22563 BAC16312 BAC34669 BAC35825 BAE01909 BAC16311.1 BAC16312.1 BAF85787.1
EMBL	CAA90215 CAB57325 CAB57326 CAB57327 CAB57328 CAF91245.1 CAN87991.1 CAN87992.1 CAF94511.1
GB	AAA37410 AAA52494 AAA52592 AAB40051 AAC00027
REF	NP_001003254 NP_001008027 NP_001017070 NP_001018130 NP_001034891 XP_001363384.1 XP_001363301.1 NP_035165.2 NP_001070366.1 XP_508657.2
SP	P60766 P60952 P60953 Q007T2 Q2KJ93
TPG	DAA32122
SWISS-PROT	Q13153 O88643 Q08E52 P35465
PRF	2003404A
GenBank	ACA13260.1 ACA13261.1 EAW75028.1 EDL16325.1 EDM18463.1
AlphaFold	P60766 P60952 P60953 Q007T2 Q2KJ93