BMRB Entry 15424

Name: NMR Assignment of Cdc42(T35A), and Active Switch I mutant of Cdc42
Published: 2007-08-16

Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR15424

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Assignment of Cdc42(T35A), and Active Switch I mutant of Cdc42

Deposition date:

2007-08-08

Original release date:

2007-08-16

Authors:

Oswald, Robert

Citation:

Citation: Adams, Paul; Oswald, Robert. "NMR assignment of Cdc42(T35A), an active Switch I mutant of Cdc42" Biomol. NMR Assignments 1, 225-227 (2007).
PubMed: 19636871

Assembly members:

Assembly members:
Cdc42(T35A), polymer, 178 residues, Formula weight is not available

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Cdc42(T35A): MQTIKCVVVGDGAVGKTCLL ISYTTNKFPSEYVPAVFDNY AVTVMIGGEPYTLGLFDTAG QEDYDRLRPLSYPQTDVFLV CFSVVSPSSFENVKEKWVPE ITHHCPKTPFLLVGTQIDLR DDPSTIEKLAKNKQKPITPE TAEKLARDLKAVKYVECSAL TQKGLKNVFDEAILAALE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	643
15N chemical shifts	172
1H chemical shifts	852

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Cdc42(T35A) monomer	1

Entities:

Entity 1, Cdc42(T35A) monomer 178 residues - Formula weight is not available

1

MET

GLN

THR

ILE

LYS

CYS

VAL

GLY

2

ASP

GLY

ALA

VAL

GLY

LYS

THR

CYS

LEU

3

ILE

SER

TYR

THR

ASN

LYS

PHE

PRO

SER

4

GLU

TYR

VAL

PRO

ALA

VAL

PHE

ASP

ASN

TYR

5

ALA

VAL

THR

VAL

MET

ILE

GLY

GLU

PRO

6

TYR

THR

LEU

GLY

LEU

PHE

ASP

THR

ALA

GLY

7

GLN

GLU

ASP

TYR

ASP

ARG

LEU

ARG

PRO

LEU

8

SER

TYR

PRO

GLN

THR

ASP

VAL

PHE

LEU

VAL

9

CYS

PHE

SER

VAL

SER

PRO

SER

PHE

10

GLU

ASN

VAL

LYS

GLU

LYS

TRP

VAL

PRO

GLU

11

ILE

THR

HIS

CYS

PRO

LYS

THR

PRO

PHE

12

LEU

VAL

GLY

THR

GLN

ILE

ASP

LEU

ARG

13

ASP

PRO

SER

THR

ILE

GLU

LYS

LEU

ALA

14

LYS

ASN

LYS

GLN

LYS

PRO

ILE

THR

PRO

GLU

15

THR

ALA

GLU

LYS

LEU

ALA

ARG

ASP

LEU

LYS

16

ALA

VAL

LYS

TYR

VAL

GLU

CYS

SER

ALA

LEU

17

THR

GLN

LYS

GLY

LEU

LYS

ASN

VAL

PHE

ASP

18

GLU

ALA

ILE

LEU

ALA

LEU

GLU

Samples:

sample_1: Cdc42(T35A), [U-100% 13C; U-100% 15N], 0.3 – 0.5 mM

sample_conditions_1: ionic strength: 0.3 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian INOVA 500 MHz

BMRB	4700
PDB	1A4R 1AJE 1AM4 1AN0 1CEE 1CF4 1DOA 1E0A 1EES 1GRN 1GZS 1KI1 1KZ7 1KZG 1NF3 2ASE 2DFK 2KB0 2NGR 2ODB 2QRZ 2WM9 2WMN 2WMO 3EG5 3GCG 3QBV 3VHL 4DID 4ITR 4JS0 4YC7 4YDH
DBJ	BAB22563 BAC16312 BAC34669 BAC35825 BAE01909
EMBL	CAA90215 CAB57325 CAB57326 CAB57327 CAB57328
GB	AAA37410 AAA52494 AAA52592 AAB40051 AAC00027
REF	NP_001003254 NP_001008027 NP_001017070 NP_001018130 NP_001034891
SP	P60766 P60952 P60953 Q007T2 Q2KJ93
TPG	DAA32122
AlphaFold	P60766 P60952 P60953 Q007T2 Q2KJ93