BMRB Entry 4599

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PDB ID: 7hsc
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4599
MolProbity Validation Chart

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

High-Resolution Solution Structure of the 18 kDa Substrate-Binding Domain of the Mammalian Chaperone Protein Hsc70

Deposition date:

2000-05-05

Original release date:

2001-02-01

Authors:

Morshauser, R.; Hu, W.; Wang, H.; Pang, Y.; Flynn, G.; Zuiderweg, E.

Citation:

Citation: Morshauser, R.; Hu, W.; Wang, H.; Pang, Y.; Flynn, G.; Zuiderweg, E.. "High-Resolution Solution Structure of the 18 kDa Substrate-Binding Domain of the Mammalian Chaperone Protein Hsc70 " J. Mol. Biol. 289, 1387-1403 (1999).

Assembly members:

Assembly members:
heat shock cognate 70 kd protein 1, polymer, 161 residues, Formula weight is not available

Natural source:

Natural source: Common Name: rat Taxonomy ID: 10116 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Rattus norvegicus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
heat shock cognate 70 kd protein 1: SENVQDLLLLDVTPLSLGIE TAGGVMTVLIKRNTTIPTKQ TQTFTTYSDNQPGVLIQVYE GERAMTKDNNLLGKFELTGI PPAPRGVPQIEVTFDIDANG ILNVSAVDKSTGKENKITIT NDKGRLSKEDIERMVQEAEK YKAEDEKQRDKVSSKNSLEH H

Data sets:

assigned_chemical_shifts
- chemical_shift_set_1

Data type	Count
1H chemical shifts	979
13C chemical shifts	657
15N chemical shifts	153

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	HSC70	1

Entities:

Entity 1, HSC70 161 residues - Formula weight is not available

1

SER

GLU

ASN

VAL

GLN

ASP

LEU

2

ASP

VAL

THR

PRO

LEU

SER

LEU

GLY

ILE

GLU

3

THR

ALA

GLY

VAL

MET

THR

VAL

LEU

ILE

4

LYS

ARG

ASN

THR

ILE

PRO

THR

LYS

GLN

5

THR

GLN

THR

PHE

THR

TYR

SER

ASP

ASN

6

GLN

PRO

GLY

VAL

LEU

ILE

GLN

VAL

TYR

GLU

7

GLY

GLU

ARG

ALA

MET

THR

LYS

ASP

ASN

8

LEU

GLY

LYS

PHE

GLU

LEU

THR

GLY

ILE

9

PRO

ALA

PRO

ARG

GLY

VAL

PRO

GLN

ILE

10

GLU

VAL

THR

PHE

ASP

ILE

ASP

ALA

ASN

GLY

11

ILE

LEU

ASN

VAL

SER

ALA

VAL

ASP

LYS

SER

12

THR

GLY

LYS

GLU

ASN

LYS

ILE

THR

ILE

THR

13

ASN

ASP

LYS

GLY

ARG

LEU

SER

LYS

GLU

ASP

14

ILE

GLU

ARG

MET

VAL

GLN

GLU

ALA

GLU

LYS

15

TYR

LYS

ALA

GLU

ASP

GLU

LYS

GLN

ARG

ASP

16

LYS

VAL

SER

LYS

ASN

SER

LEU

GLU

HIS

17

HIS

Samples:

sample_1: heat shock cognate 70 kd protein 1, [U-15N], 1.5 mM; sodium phosphate 50 mM; D2O 5%; H2O 95%

sample_2: heat shock cognate 70 kd protein 1, [U-15N; U-13C], 1.5 mM; sodium phosphate 50 mM; D2O 5%; H2O 95%

sample_3: heat shock cognate 70 kd protein 1, [U-15N], 1.5 mM; sodium phosphate 50 mM; D2O 100%

sample_4: heat shock cognate 70 kd protein 1, [U-15N; U-13C], 1.5 mM; sodium phosphate 50 mM; D2O 100%

sample_cond_1: pH: 7.0; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
HNCA	not available	not available	sample_cond_1
HN(CA)HA	not available	not available	sample_cond_1
HN(CO)CA	not available	not available	sample_cond_1
HA(CACO)NH	not available	not available	sample_cond_1
CP H(C)CCH-TOCSY	not available	not available	sample_cond_1
CP (H)CCH-TOCSY	not available	not available	sample_cond_1
CP(H)C(CCACO)NH-TOCSY	not available	not available	sample_cond_1
15N RESOLVED NOESY-HSQC	not available	not available	sample_cond_1
13C RESOLVED NOESY-HMQC	not available	not available	sample_cond_1
4D 13C-13C RESOLVED HMQC-NOESY	not available	not available	sample_cond_1
3D 13C-13N RESOLVED HMQC-NOESY-HSQC	not available	not available	sample_cond_1
3D 13C-13C RESOLVED HMQC-NOESY-HSQC-HSQC	not available	not available	sample_cond_1

Software:

DISCOVER - refinement

FELIX - structure calculation

MSI INSIGHT vINSIGHT - structure calculation

MSI DISCOVER vDISCOVER - structure calculation

NMR spectrometers:

BRUKER DMX 750 MHz

BMRB	4497
PDB	1CKR 1YUW 2V7Z 3C7N 4FL9 7HSC
DBJ	BAB69718 BAC29016 BAC36065 BAD12572 BAD96505
EMBL	CAA06233 CAA37422 CAA37823 CAA49670 CAA68265
GB	AAA36991 AAA37869 AAA41354 AAB18391 AAC52836
PIR	S31716
REF	NP_001075247 NP_001079632 NP_001080068 NP_001091238 NP_001125783
SP	A2Q0Z1 O73885 P11142 P19120 P19378
TPG	DAA22199 DAA34064
AlphaFold	A2Q0Z1 O73885 P11142 P19120 P19378