BMRB Entry 4497

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4497
MolProbity Validation Chart

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Title:
High resolution solution structure of the Heat shock cognate -70 kd substrate binding domain obtained by multidimensional NMR techniques
Deposition date:
1999-05-04
Original release date:
2000-06-16
Authors:
MORSHAUSER, R.; HU, W.; WANG, H.; PANG, Y.; FLYNN, G.; ZUIDERWEG, E.
Citation:

Citation: MORSHAUSER, R.; HU, W.; WANG, H.; PANG, Y.; FLYNN, G.; ZUIDERWEG, E.. "High-resolution solution structure of the 18 kDa substrate-binding domain of the mammalian chaperone protein Hsc70"  J. Mol. Biol. 289, 1387-1403 (1999).
PubMed: 10373374

Assembly members:

Assembly members:
Heat shock cognate 70 kd protein 1, polymer, 161 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Norway rat   Taxonomy ID: 10116   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Rattus norvegicus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Heat shock cognate 70 kd protein 1: SENVQDLLLLDVTPLSLGIE TAGGVMTVLIKRNTTIPTKQ TQTFTTYSDNQPGVLIQVYE GERAMTKDNNLLGKFELTGI PPAPRGVPQIEVTFDIDANG ILNVSAVDKSTGKENKITIT NDKGRLSKEDIERMVQEAEK YKAEDEKQRDKVSSKNSLEH H

Data sets:
Data typeCount
1H chemical shifts1065
13C chemical shifts683
15N chemical shifts153

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1HSP701

Entities:

Entity 1, HSP70 161 residues - Formula weight is not available

1   SERGLUASNVALGLNASPLEULEULEULEU
2   ASPVALTHRPROLEUSERLEUGLYILEGLU
3   THRALAGLYGLYVALMETTHRVALLEUILE
4   LYSARGASNTHRTHRILEPROTHRLYSGLN
5   THRGLNTHRPHETHRTHRTYRSERASPASN
6   GLNPROGLYVALLEUILEGLNVALTYRGLU
7   GLYGLUARGALAMETTHRLYSASPASNASN
8   LEULEUGLYLYSPHEGLULEUTHRGLYILE
9   PROPROALAPROARGGLYVALPROGLNILE
10   GLUVALTHRPHEASPILEASPALAASNGLY
11   ILELEUASNVALSERALAVALASPLYSSER
12   THRGLYLYSGLUASNLYSILETHRILETHR
13   ASNASPLYSGLYARGLEUSERLYSGLUASP
14   ILEGLUARGMETVALGLNGLUALAGLULYS
15   TYRLYSALAGLUASPGLULYSGLNARGASP
16   LYSVALSERSERLYSASNSERLEUGLUHIS
17   HIS

Samples:

sample_1: Heat shock cognate 70 kd protein 1 mM; sodium Phosphate 50 mM

sample_cond_1: pH: 7.0; temperature: 298 K; ionic strength: 50 mM; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
HNCAsample_1not availablesample_cond_1
HN(CA)HAsample_1not availablesample_cond_1
HN(CO)CAsample_1not availablesample_cond_1
HA(CACO)NHsample_1not availablesample_cond_1
CP H(C)CCH-TOCSYsample_1not availablesample_cond_1
CP (H)CCH-TOCSYsample_1not availablesample_cond_1
CP(H)C(CCACO)NH-TOCSYsample_1not availablesample_cond_1
15N-RESOLVED NOESY-HSQCsample_1not availablesample_cond_1
13CRESOLVED NOESY-HMQCsample_1not availablesample_cond_1
4D 13C,13C RESOLVED HMQC-NOESYsample_1not availablesample_cond_1
3D 13C,13N RESOLVED HMQC-NOESY-HSQCsample_1not availablesample_cond_1
3D 13C,13C RESOLVED HMQC-NOESY-HSQC,-HSQCsample_1not availablesample_cond_1

Software:

No software information available

NMR spectrometers:

  • Bruker AMX 500 MHz
  • Bruker AMX 600 MHz
  • Bruker DMX 750 MHz
  • Bruker DMX 500 MHz
  • Bruker DMX 600 MHz

Related Database Links:

BMRB 4599
PDB
DBJ BAB69718 BAC29016 BAC36065 BAD12572 BAD96505
EMBL CAA06233 CAA37422 CAA37823 CAA49670 CAA68265
GB AAA36991 AAA37869 AAA41354 AAB18391 AAC52836
PIR S31716
REF NP_001075247 NP_001079632 NP_001080068 NP_001091238 NP_001125783
SP A2Q0Z1 O73885 P11142 P19120 P19378
TPG DAA22199 DAA34064
AlphaFold A2Q0Z1 O73885 P11142 P19120 P19378

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks