BMRB Entry 4514

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4514
MolProbity Validation Chart

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Title:
NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein (LRP). Evidence for specific binding to the receptor binding domain of human alpha-2 macroglobulin
Deposition date:
1999-10-08
Original release date:
2001-05-07
Authors:
Dolmer, K.; Huang, W.; Gettins, P.
Citation:

Citation: Dolmer, K.; Huang, W.; Gettins, P.. "NMR solution structure of complement-like repeat CR3 from the low density lipoprotein receptor-related protein (LRP). Evidence for specific binding to the receptor binding domain of human alpha-2 macroglobulin"  J. Biol. Chem. 275, 3264-3269 (2000).
PubMed: 10652313

Assembly members:

Assembly members:
lipoprotein receptor related protein, polymer, 45 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Vector: PGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
lipoprotein receptor related protein: GSPPQCQPGEFACANSRCIQ ERWKCDGDNDCLDNSDEAPA LCHQH

Data sets:
Data typeCount
13C chemical shifts69
15N chemical shifts40
1H chemical shifts220

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1lipoprotein receptor related protein1
2Calcium ion2

Entities:

Entity 1, lipoprotein receptor related protein 45 residues - Formula weight is not available

1   GLYSERPROPROGLNCYSGLNPROGLYGLU
2   PHEALACYSALAASNSERARGCYSILEGLN
3   GLUARGTRPLYSCYSASPGLYASPASNASP
4   CYSLEUASPASNSERASPGLUALAPROALA
5   LEUCYSHISGLNHIS

Entity 2, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: lipoprotein receptor related protein, [U-15N; U-13C], 2 mM; sodium acetate, [U-2H], 20 mM; sodium chloride 10 mM; H2O 90%; D2O 10%

sample_cond_1: ionic strength: 30 mM; pH: 5.5; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
3D 15N-SEPARATED TOCSYsample_1not availablesample_cond_1
3D 13C-SEPARATED TOCSYsample_1not availablesample_cond_1
3D 15N-SEPARATED NOESYsample_1not availablesample_cond_1
3D 13C-SEPARATED NOESYsample_1not availablesample_cond_1
HBHA(CO)NHsample_1not availablesample_cond_1
CBCA(CO)NHsample_1not availablesample_cond_1

Software:

SYBYL v6.3 - processing

DYANA v1.5 - structure solution

AMBER v5.0 - refinement

PROFIT v1.8 - structure solution

NMR spectrometers:

  • BRUKER DRX 600 MHz

Related Database Links:

BMRB 16454
PDB

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