BMRB Entry 16454

Title:
NMR Solution Structure of Complement-like Repeat CR3 from the Low Density Lipoprotein REceptor-related Protein
Deposition date:
2009-08-19
Original release date:
2010-03-08
Authors:
Dolmer, Klavs; Huang, Wen; Gettins, Peter
Citation:

Citation: Dolmer, Klavs; Huang, Wen; Gettins, Peter. "NMR Solution Structure of Complement-like Repeat CR3 from the Low Density Lipoprotein Receptor-related Protein"  J. Biol. Chem. 275, 3264-3269 (2000).
PubMed: 10652313

Assembly members:

Assembly members:
CR3, polymer, 41 residues, Formula weight is not available
RBD, polymer, 357 residues, Formula weight is not available
CA, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Pseudomonas   Taxonomy ID: 286   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Pseudomonas not available

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: homo sapiens   Vector: na

Data sets:
  • binding_constants
Data typeCount
binding constants1

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1CR31
2RBD2
3Calcium ion3

Entities:

Entity 1, CR3 41 residues - Formula weight is not available

1   GLNCYSGLNPROGLYGLUPHEALACYSALA
2   ASNSERARGCYSILEGLNGLUARGTRPLYS
3   CYSASPGLYASPASNASPCYSLEUASPASN
4   SERASPGLUALAPROALALEUCYSHISGLN
5   HIS

Entity 2, RBD 357 residues - Formula weight is not available

1   METALAPROARGARGVALARGSERPHELEU
2   ARGGLYLEUPROALALEULEULEULEULEU
3   LEUPHELEUGLYPROTRPPROALAALASER
4   HISGLYGLYLYSTYRSERARGGLULYSASN
5   GLNPROLYSPROSERPROLYSARGGLUSER
6   GLYGLUGLUPHEARGMETGLULYSLEUASN
7   GLNLEUTRPGLULYSALAGLNARGLEUHIS
8   LEUPROPROTRPARGLEUALAGLULEUHIS
9   ALAASPLEULYSILEGLNGLUARGASPGLU
10   LEUALATRPLYSLYSLEULYSLEUASPGLY
11   LEUASPGLUASPGLYGLULYSGLUALAARG
12   LEUILEARGASNLEUASNVALILELEUALA
13   LYSTYRGLYLEUASPGLYLYSLYSASPALA
14   ARGGLNVALTHRSERASNSERLEUSERGLY
15   THRGLNGLUASPGLYLEUASPASPPROARG
16   LEUGLULYSLEUTRPHISLYSALALYSTHR
17   SERGLYLYSPHESERGLYGLUGLULEUASP
18   LYSLEUTRPARGGLUPHELEUHISHISLYS
19   GLULYSVALHISGLUTYRASNVALLEULEU
20   GLUTHRLEUSERARGTHRGLUGLUILEHIS
21   GLUASNVALILESERPROSERASPLEUSER
22   ASPILELYSGLYSERVALLEUHISSERARG
23   HISTHRGLULEULYSGLULYSLEUARGSER
24   ILEASNGLNGLYLEUASPARGLEUARGARG
25   VALSERHISGLNGLYTYRSERTHRGLUALA
26   GLUPHEGLUGLUPROARGVALILEASPLEU
27   TRPASPLEUALAGLNSERALAASNLEUTHR
28   ASPLYSGLULEUGLUALAPHEARGGLUGLU
29   LEULYSHISPHEGLUALALYSILEGLULYS
30   HISASNHISTYRGLNLYSGLNLEUGLUILE
31   ALAHISGLULYSLEUARGHISALAGLUSER
32   VALGLYASPGLYGLUARGVALSERARGSER
33   ARGGLULYSHISALALEULEUGLUGLYARG
34   THRLYSGLULEUGLYTYRTHRVALLYSLYS
35   HISLEUGLNASPLEUSERGLYARGILESER
36   ARGALAARGHISASNGLULEU

Entity 3, Calcium ion - Ca - 40.078 Da.

1   CA

Samples:

sample_1: CR3, [U-13C; U-15N], 100 uM; Tris-HCl 20 mM; sodium chloride 100 mM; Ca2+ 10 mM; sodium azide 0.1%; RBD0 – 400 uM; H2O 90%; D2O 10%

sample_conditions_1: pH: 7.4; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1

Software:

Triad v6.3, (Tripos, Inc., ST. Louis, MO) - processing

DYANA v1.5, Guntert, Braun and Wuthrich - structure solution

AMBER v5.0, Case, Darden, Cheatham, III, Simmerling, Wang, Duke, Luo, ... and Kollm - structure solution

NMR spectrometers:

  • Bruker DRX 600 MHz

Related Database Links:

BMRB 4514
PDB
DBJ BAD96224 BAD96247 BAF85010 BAG35576 BAG73353
EMBL CAH90295
GB AAA51553 AAC67373 AAI05075 AAI12068 AAY18903
REF NP_001125136 NP_001233320 NP_002328 XP_003804417 XP_004038406
SP P30533
AlphaFold P30533