BMRB Entry 4306

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4306

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone NMR asignment of a cyanobacterial transcriptional factor, SmtB, that has bound zinc ions

Deposition date:

1999-02-03

Original release date:

2000-07-14

Authors:

Kosada, Takashi; Morita, Hayato; Miura, Akira; Yamazaki, Toshio; Hayashi, Hidenori; Kyogoku, Yoshimasa

Citation:

Citation: Kosada, Takashi; Morita, Hayato; Miura, Akira; Yamazaki, Toshio; Hayashi, Hidenori; Kyogoku, Yoshimasa. "Backbone NMR assignments of a cyanobacterial transcriptional factor, SmtB, that binds zinc ions" J. Biomol. NMR 14, 191-192 (1999).

Assembly members:

Assembly members:
transcriptional repressor, polymer, 121 residues, 13395.2 Da.
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: not available Taxonomy ID: not available Superkingdom: not available Kingdom: not available Genus/species: not available not available

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: T-21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
transcriptional repressor: TKPVLQDGETVVCQGTHAAI ASELQAIAPEVAQSLAEFFA VLADPNRLRLLSLLARSELC VGDLAQAIGVSESAVSHQLR SLRNLRLVSYRKQGRHVYYQ LQDHHIVALYQNALDHLQEC R

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment_SmtB

Data type	Count
13C chemical shifts	119
15N chemical shifts	114
1H chemical shifts	114

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SmtB subunit 1	1
2	SmtB subunit 2	1
3	Zn1	2
4	Zn2	2
5	Zn3	2
6	Zn4	2

Entities:

Entity 1, SmtB subunit 1 121 residues - 13395.2 Da.

1

THR

LYS

PRO

VAL

LEU

GLN

ASP

GLY

GLU

THR

2

VAL

CYS

GLN

GLY

THR

HIS

ALA

ILE

3

ALA

SER

GLU

LEU

GLN

ALA

ILE

ALA

PRO

GLU

4

VAL

ALA

GLN

SER

LEU

ALA

GLU

PHE

ALA

5

VAL

LEU

ALA

ASP

PRO

ASN

ARG

LEU

ARG

LEU

6

LEU

SER

LEU

ALA

ARG

SER

GLU

LEU

CYS

7

VAL

GLY

ASP

LEU

ALA

GLN

ALA

ILE

GLY

VAL

8

SER

GLU

SER

ALA

VAL

SER

HIS

GLN

LEU

ARG

9

SER

LEU

ARG

ASN

LEU

ARG

LEU

VAL

SER

TYR

10

ARG

LYS

GLN

GLY

ARG

HIS

VAL

TYR

GLN

11

LEU

GLN

ASP

HIS

ILE

VAL

ALA

LEU

TYR

12

GLN

ASN

ALA

LEU

ASP

HIS

LEU

GLN

GLU

CYS

13

ARG

Entity 2, Zn1 - Zn - 65.409 Da.

1

ZN

Samples:

sample_one: transcriptional repressor, [U-15N; U-13C], 0.6 mM; H2O 90%; D2O 10%

sample_two: transcriptional repressor, [U-15N]-A,V,L,H,R, 0.6 mM; H2O 90%; D2O 10%

Ex-cond_1: pH: 6.0; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	not available	not available	Ex-cond_1
3D HNCACB	not available	not available	Ex-cond_1
3D CBCA(CO)NH	not available	not available	Ex-cond_1
3D HNCA	not available	not available	Ex-cond_1
3D HN(CO)CA	not available	not available	Ex-cond_1

Software:

NMR-PIPE v1.6 - peak picking and assignments

NMR spectrometers:

Bruker DMX 500 MHz

BMRB	4128
PDB	1R1T 1R22 1R23 1SMT
DBJ	BAD78452
EMBL	CAA45872
GB	AAT01609 AAT01610 AAT01611 AAT01612 AAT01613
REF	WP_011242576
SP	P30340
AlphaFold	P30340