BMRB Entry 4128

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4128

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR Backbone Assignments of the Cyanobacterial Transcriptional Factor, SmtB, that Senses the Zinc Concentration in the Cell

Deposition date:

1998-03-23

Original release date:

1999-02-03

Authors:

Morita, Hayato; Kosada, Takashi; Yamazaki, Toshio; Kyogoku, Yoshimasa; Hayashi, Hidenori

Citation:

Citation: Morita, Hayato; Kosada, Takashi; Yamazaki, Toshio; Kyogoku, Yoshimasa; Hayashi, Hidenori. "NMR Backbone Assignments of the Cyanobacterial Transcriptional Factor, SmtB, that Senses the Zinc Concentration in the Cell" J. Biomol. NMR 12, 453-454 (1998).

Assembly members:

Assembly members:
cyanobacterial metallothionein gene repressor, polymer, 121 residues, 13395.2 Da.

Natural source:

Natural source: Common Name: Synechococcus Taxonomy ID: 1140 Superkingdom: Eubacteria Kingdom: not available Genus/species: Synechococcus PCC7942

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET-21d

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cyanobacterial metallothionein gene repressor: TKPVLQDGETVVCQGTHAAI ASELQAIAPEVAQSLAEFFA VLADPNRLRLLSLLARSELC VGDLAQAIGVSESAVSHQLR SLRNLRLVSYRKQGRHVYYQ LQDHHIVALYQNALDHLQEC R

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_one

Data type	Count
13C chemical shifts	119
15N chemical shifts	116
1H chemical shifts	116

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	SmtB subunit one	1
2	SmtB subunit two	1

Entities:

Entity 1, SmtB subunit one 121 residues - 13395.2 Da.

1

THR

LYS

PRO

VAL

LEU

GLN

ASP

GLY

GLU

THR

2

VAL

CYS

GLN

GLY

THR

HIS

ALA

ILE

3

ALA

SER

GLU

LEU

GLN

ALA

ILE

ALA

PRO

GLU

4

VAL

ALA

GLN

SER

LEU

ALA

GLU

PHE

ALA

5

VAL

LEU

ALA

ASP

PRO

ASN

ARG

LEU

ARG

LEU

6

LEU

SER

LEU

ALA

ARG

SER

GLU

LEU

CYS

7

VAL

GLY

ASP

LEU

ALA

GLN

ALA

ILE

GLY

VAL

8

SER

GLU

SER

ALA

VAL

SER

HIS

GLN

LEU

ARG

9

SER

LEU

ARG

ASN

LEU

ARG

LEU

VAL

SER

TYR

10

ARG

LYS

GLN

GLY

ARG

HIS

VAL

TYR

GLN

11

LEU

GLN

ASP

HIS

ILE

VAL

ALA

LEU

TYR

12

GLN

ASN

ALA

LEU

ASP

HIS

LEU

GLN

GLU

CYS

13

ARG

Samples:

sample_one: cyanobacterial metallothionein gene repressor, [U-15N];[U-13C,U-15N];[15N]-His;[15N]-Lys;[15N]-Arg;[15N]-Val;[15N]-Leu;[15N]-Cys, 0.6 mM; H2O 90%; D2O 10%

sample_conditions_one: pH: 6.0; temperature: 313 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D_1H-15N_HSQC	sample_one	not available	sample_conditions_one
Three-dimensional experiments	sample_one	not available	sample_conditions_one
3D_HNCACB	sample_one	not available	sample_conditions_one
3D_CBCA(CO)NH	sample_one	not available	sample_conditions_one
3D_HNCA	sample_one	not available	sample_conditions_one
3D_HN(CO)CA	sample_one	not available	sample_conditions_one

Software:

NMRPipe v1.6, NIH - peak picking and assignments

NMR spectrometers:

Bruker DMX 500 MHz

BMRB	4306
PDB	1R1T 1R22 1R23 1SMT
DBJ	BAD78452
EMBL	CAA45872
GB	AAT01609 AAT01610 AAT01611 AAT01612 AAT01613
REF	WP_011242576
SP	P30340
AlphaFold	P30340