BMRB Entry 4300

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4300
MolProbity Validation Chart

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Title:
1H , 13C, 15N Assigned Chemical Shifts for HPPK-AMPPCP Complex
Deposition date:
1999-01-22
Original release date:
1999-08-12
Authors:
Shi, Genbin; Gao, Jinhai; Yan, Honggao
Citation:

Citation: Shi, Genbin; Gao, Jinhai; Yan, Honggao. "1H, 13C and 15N Resonance Assignments of Escherichia coli 6-hydroxymethyl-7,8-dihydropterin Pyrophosphokinase and its Complex with MgAMPPCP"  J. Biomol. NMR 14, 189-190 (1999).

Assembly members:

Assembly members:
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, polymer, 158 residues, Formula weight is not available
beta gamma-methyleneadenosine 5'triphosphate, non-polymer, Formula weight is not available
MAGNESIUM ION, non-polymer, 24.305 Da.

Natural source:

Natural source:   Common Name: E.coli   Taxonomy ID: 562   Superkingdom: Eubacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-17B

Entity Sequences (FASTA):

Entity Sequences (FASTA):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: TVAYIAIGSNLASPLEQVNA ALKALGDIPESHILTVSSFY RTPPLGPQDQPDYLNAAVAL ETSLAPEELLNHTQRIELQQ GRVRKAERWGPRTLDLDIML FGNEVINTERLTVPHYDMKN RGFMLWPLFEIAPELVFPDG EMLRQILHTRAFDKLNKW

Data sets:
Data typeCount
1H chemical shifts868
13C chemical shifts240
15N chemical shifts139

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1HPPK monomer1
2AMPPCP2
3Mg3

Entities:

Entity 1, HPPK monomer 158 residues - Formula weight is not available

1   THRVALALATYRILEALAILEGLYSERASN
2   LEUALASERPROLEUGLUGLNVALASNALA
3   ALALEULYSALALEUGLYASPILEPROGLU
4   SERHISILELEUTHRVALSERSERPHETYR
5   ARGTHRPROPROLEUGLYPROGLNASPGLN
6   PROASPTYRLEUASNALAALAVALALALEU
7   GLUTHRSERLEUALAPROGLUGLULEULEU
8   ASNHISTHRGLNARGILEGLULEUGLNGLN
9   GLYARGVALARGLYSALAGLUARGTRPGLY
10   PROARGTHRLEUASPLEUASPILEMETLEU
11   PHEGLYASNGLUVALILEASNTHRGLUARG
12   LEUTHRVALPROHISTYRASPMETLYSASN
13   ARGGLYPHEMETLEUTRPPROLEUPHEGLU
14   ILEALAPROGLULEUVALPHEPROASPGLY
15   GLUMETLEUARGGLNILELEUHISTHRARG
16   ALAPHEASPLYSLEUASNLYSTRP

Entity 2, AMPPCP 1 residues - C11H16N5O12P3Na2 - Formula weight is not available

1   AMPPCP

Entity 3, Mg - Mg - 24.305 Da.

1   MG

Samples:

sample_1: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, [U-15N], 1.5 mM; sodium phosphate 20 mM; B,r-Methyleneadenosine 5`-triphosphate 8 mM; Magnesium chloride 40 mM; H2O 90%; D2O 10%

sample_2: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, [U-15N; U-13C], 1.5 mM; sodium phosphate 20 mM; B,r-Methyleneadenosine 5`-triphosphate 8 mM; Magnesium chloride 40 mM; H2O 90%; D2O 10%

sample_conditions_1: pH*: 7.4 na; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availablesample_conditions_1

Software:

No software information available

NMR spectrometers:

  • . . . MHz

Related Database Links:

BMRB 4299
PDB
DBJ BAB96719 BAJ41992 BAL37452
EMBL CBI99639 CCP97244 CDJ70723 CDK83585 CDY54783
GB AAB53446 AAC73253 ACA79131 ACB01321 ACR61761
REF NP_414684 WP_000215127 WP_000215131 WP_000215137 WP_000215138
SP P26281
AlphaFold P26281

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