BMRB Entry 4299
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4299
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Shi, Genbin; Gao, Jinhai; Yan, Honggao. "1H, 13C and 15N Resonance Assignments of Escherichia coli
6-hydroxymethyl-7,8-dihydropterin Pyrophosphokinase and its
Complex with MgAMPPCP" J. Biomol. NMR 14, 189-190 (1999).
Assembly members:
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, polymer, 158 residues, Formula weight is not available
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Eubacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: plasmid pET-17B
Entity Sequences (FASTA):
6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase: TVAYIAIGSNLASPLEQVNA
ALKALGDIPESHILTVSSFY
RTPPLGPQDQPDYLNAAVAL
ETSLAPEELLNHTQRIELQQ
GRVRKAERWGPRTLDLDIML
FGNEVINTERLTVPHYDMKN
RGFMLWPLFEIAPELVFPDG
EMLRQILHTRAFDKLNKW
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 240 |
| 15N chemical shifts | 127 |
| 1H chemical shifts | 858 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | HPPK_monomer | 1 |
Entities:
Entity 1, HPPK_monomer 158 residues - Formula weight is not available
| 1 | THR | VAL | ALA | TYR | ILE | ALA | ILE | GLY | SER | ASN | ||||
| 2 | LEU | ALA | SER | PRO | LEU | GLU | GLN | VAL | ASN | ALA | ||||
| 3 | ALA | LEU | LYS | ALA | LEU | GLY | ASP | ILE | PRO | GLU | ||||
| 4 | SER | HIS | ILE | LEU | THR | VAL | SER | SER | PHE | TYR | ||||
| 5 | ARG | THR | PRO | PRO | LEU | GLY | PRO | GLN | ASP | GLN | ||||
| 6 | PRO | ASP | TYR | LEU | ASN | ALA | ALA | VAL | ALA | LEU | ||||
| 7 | GLU | THR | SER | LEU | ALA | PRO | GLU | GLU | LEU | LEU | ||||
| 8 | ASN | HIS | THR | GLN | ARG | ILE | GLU | LEU | GLN | GLN | ||||
| 9 | GLY | ARG | VAL | ARG | LYS | ALA | GLU | ARG | TRP | GLY | ||||
| 10 | PRO | ARG | THR | LEU | ASP | LEU | ASP | ILE | MET | LEU | ||||
| 11 | PHE | GLY | ASN | GLU | VAL | ILE | ASN | THR | GLU | ARG | ||||
| 12 | LEU | THR | VAL | PRO | HIS | TYR | ASP | MET | LYS | ASN | ||||
| 13 | ARG | GLY | PHE | MET | LEU | TRP | PRO | LEU | PHE | GLU | ||||
| 14 | ILE | ALA | PRO | GLU | LEU | VAL | PHE | PRO | ASP | GLY | ||||
| 15 | GLU | MET | LEU | ARG | GLN | ILE | LEU | HIS | THR | ARG | ||||
| 16 | ALA | PHE | ASP | LYS | LEU | ASN | LYS | TRP |
Samples:
sample_one: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, [U-15N], 1.5 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%
sample_two: 6-hydroxymethyl-7,8-dihydropterin pyrophosphokinase, [U-15N;U-13C], 1.5 mM; sodium phosphate 20 mM; H2O 90%; D2O 10%
sample_conditions_one: pH: 7.4; temperature: 298 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|
Software:
No software information available
NMR spectrometers:
- unknown unknown 0 MHz
Related Database Links:
| BMRB | 4300 |
| PDB | |
| DBJ | BAB96719 BAJ41992 BAL37452 |
| EMBL | CBI99639 CCP97244 CDJ70723 CDK83585 CDY54783 |
| GB | AAB53446 AAC73253 ACA79131 ACB01321 ACR61761 |
| REF | NP_414684 WP_000215127 WP_000215131 WP_000215137 WP_000215138 |
| SP | P26281 |
| AlphaFold | P26281 |
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks