BMRB Entry 4204

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4204
MolProbity Validation Chart

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Title:
Three-Dimensional Structure of Human Insulin-Like Growth Factor-I (IGF-I) Determined by 1H-NMR and Distance Geometry
Deposition date:
1997-09-21
Original release date:
1999-12-22
Authors:
Sato, A.; Nishimura, S.; Ohkubo, T.; Kyogoku, Y.; Koyama, S.; Kobayashi, M.; Kobayashi, T.
Citation:

Citation: Sato, A.; Nishumura, S.; Ohkubo, T.; Kyogoku, Y.; Koyama, S.; Kobatashi, M.; Yasuda, T.; Kobayashi, Y.. "Three-Dimensional Structure of Human Insulin-like Growth Factor-I (IGF-I) Determined by 1H-NMR and Distance Geometry"  Int. J. Pept. Protein Res. 41, 433-440 (1993).

Assembly members:

Assembly members:
Insulin-like growth factor-I (Somatomedin C), polymer, 70 residues, 7649 Da.

Natural source:

Natural source:   Common Name: human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Insulin-like growth factor-I (Somatomedin C): GPETLCGAELVDALQFVCGD RGFYFNKPTGYGSSSRRAPQ TGIVDECCFRSCDLRRLEMY CAPLKPAKSA

Data sets:
Data typeCount
1H chemical shifts459

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1IGF-I1

Entities:

Entity 1, IGF-I 70 residues - 7649 Da.

1   GLYPROGLUTHRLEUCYSGLYALAGLULEU
2   VALASPALALEUGLNPHEVALCYSGLYASP
3   ARGGLYPHETYRPHEASNLYSPROTHRGLY
4   TYRGLYSERSERSERARGARGALAPROGLN
5   THRGLYILEVALASPGLUCYSCYSPHEARG
6   SERCYSASPLEUARGARGLEUGLUMETTYR
7   CYSALAPROLEULYSPROALALYSSERALA

Samples:

sample_one: Insulin-like growth factor-I (Somatomedin C) 10 mM; D20 90%; acetic acid, [U-2H], 10%

sample_two: Insulin-like growth factor-I (Somatomedin C) 10 mM; D20 10%; H20 80%; acetic acid, [U-2H], 10%

sample_conditions_one: pH: 3.0; temperature: 313 K

Experiments:

NameSampleSample stateSample conditions
ph dependence experimentsample_onenot availablesample_conditions_one
temperature dependence experimentsample_onenot availablesample_conditions_one
H/D exchange experimentsample_onenot availablesample_conditions_one

Software:

No software information available

NMR spectrometers:

  • JEOL GSX 500 MHz

Related Database Links:

BMRB 15654 2498 4069 4278 4494
PDB
DBJ BAA01200 BAA01976 BAA07897 BAB77524 BAD92421
EMBL CAA24998 CAA27153 CAA28759 CAA33746 CAA35098
GB AAA31043 AAA31544 AAA31545 AAA31546 AAA31547
PIR S22878
PRF 0912651A 1001199A 1203258A 1707201A 2001274A
REF NP_000609 NP_001009774 NP_001071296 NP_001075495 NP_001075967
SP P05019 P07455 P10763 P16545 P17647
TPG DAA29731
AlphaFold P05019 P07455 P10763 P16545 P17647