BMRB Entry 15654

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR15654

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Title:
NMR Chemical Shift Assignments of Insulin-like Growth Factor-I (IGF-I)
Deposition date:
2008-02-08
Original release date:
2009-02-09
Authors:
Kuang, Zhihe; Yao, Shenggen; McNeil, Kerrie; Forbes, Briony; Wallace, John; Norton, Ray
Citation:

Citation: Kuang, Zhihe; Yao, Shenggen; McNeil, Kerrie; Forbes, Briony; Wallace, John; Norton, Raymond. "Insulin-like growth factor-I (IGF-I): Solution properties and NMR chemical shift assignments near physiological pH."  Growth Horm. IGF Res. 19, 226-231 (2009).
PubMed: 19056307

Assembly members:

Assembly members:
Insulin-like_growth_factor-I_(IGF-I), polymer, 70 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGH(1-11)-VN-IGF-I

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Insulin-like_growth_factor-I_(IGF-I): GPETLCGAELVDALQFVCGD RGFYFNKPTGYGSSSRRAPQ TGIVDECCFRSCDLRRLEMY CAPLKPAKSA

Data sets:
Data typeCount
13C chemical shifts192
15N chemical shifts62
1H chemical shifts259

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Insulin-like growth factor-I (IGF-I)1

Entities:

Entity 1, Insulin-like growth factor-I (IGF-I) 70 residues - Formula weight is not available

1   GLYPROGLUTHRLEUCYSGLYALAGLULEU
2   VALASPALALEUGLNPHEVALCYSGLYASP
3   ARGGLYPHETYRPHEASNLYSPROTHRGLY
4   TYRGLYSERSERSERARGARGALAPROGLN
5   THRGLYILEVALASPGLUCYSCYSPHEARG
6   SERCYSASPLEUARGARGLEUGLUMETTYR
7   CYSALAPROLEULYSPROALALYSSERALA

Samples:

sample_1: Insulin-like growth factor-I (IGF-I), [U-98% 13C; U-98% 15N], 0.7 mM; H2O 95%; D2O, [U-100% 2H], 5%; sodium acetate 10 mM; sodium azide 0.02% w/v

sample_conditions_1: pH: 6.0; pressure: 1 atm; temperature: 310 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D CNH-NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

XEASY, Bartels et al. - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 500 MHz

Related Database Links:

BMRB 2498 4069 4204 4278 4494
PDB
DBJ BAA01200 BAA01976 BAA07897 BAB77524 BAD92421
EMBL CAA24998 CAA27153 CAA28759 CAA33746 CAA35098
GB AAA31043 AAA31544 AAA31545 AAA31546 AAA31547
PIR S22878
PRF 0912651A 1001199A 1203258A 1707201A 2001274A
REF NP_000609 NP_001009774 NP_001071296 NP_001075495 NP_001075967
SP P05019 P07455 P10763 P16545 P17647
TPG DAA29731
AlphaFold P05019 P07455 P10763 P16545 P17647

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks