BMRB Entry 4091

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR4091

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy

Deposition date:

1998-01-06

Original release date:

1998-03-14

Authors:

Moy, Franklin; Seddon, Andrew; Campbell, Ernest; Bohlen, Peter; Powers, Robert

Citation:

Citation: Moy, Franklin; Seddon, Andrew; Campbell, Ernest; Bohlen, Peter; Powers, Robert. "1H, 15N, 13C, and 13CO Assignments and Secondary Structure Determination of Basic Fibroblast Growth Factor using 3D Heteronuclear NMR Spectroscopy" J. Biomol. NMR 6, 245-254 (1995).

Assembly members:

Assembly members:
basic fibroblast Growth Factor, polymer, 154 residues, 17200 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: not available Superkingdom: eucaryote Kingdom: Animalia Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli

Entity Sequences (FASTA):

Entity Sequences (FASTA):
basic fibroblast Growth Factor: AEGEITTLPALPEDGGSGAF PPGHFKDPKRLYCKNGGFFL RIHPDGRVDGVREKSDPHIK LQLQAEERGVVSIKGVSANR YLAMKEDGRLLASKSVTDEC FFFERLESNNYNTYRSRKYT SWYVALKRTGQYKLGSKTGP GQKAILFLPMSAKS

Data sets:

assigned_chemical_shifts
- chemical_shift_assignment

Data type	Count
13C chemical shifts	686
15N chemical shifts	151
1H chemical shifts	1060

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FGF-2	1

Entities:

Entity 1, FGF-2 154 residues - 17200 Da.

1

ALA

GLU

GLY

GLU

ILE

THR

LEU

PRO

ALA

2

LEU

PRO

GLU

ASP

GLY

SER

GLY

ALA

PHE

3

PRO

GLY

HIS

PHE

LYS

ASP

PRO

LYS

ARG

4

LEU

TYR

CYS

LYS

ASN

GLY

PHE

LEU

5

ARG

ILE

HIS

PRO

ASP

GLY

ARG

VAL

ASP

GLY

6

VAL

ARG

GLU

LYS

SER

ASP

PRO

HIS

ILE

LYS

7

LEU

GLN

LEU

GLN

ALA

GLU

ARG

GLY

VAL

8

VAL

SER

ILE

LYS

GLY

VAL

SER

ALA

ASN

ARG

9

TYR

LEU

ALA

MET

LYS

GLU

ASP

GLY

ARG

LEU

10

LEU

ALA

SER

LYS

SER

VAL

THR

ASP

GLU

CYS

11

PHE

GLU

ARG

LEU

GLU

SER

ASN

12

TYR

ASN

THR

TYR

ARG

SER

ARG

LYS

TYR

THR

13

SER

TRP

TYR

VAL

ALA

LEU

LYS

ARG

THR

GLY

14

GLN

TYR

LYS

LEU

GLY

SER

LYS

THR

GLY

PRO

15

GLY

GLN

LYS

ALA

ILE

LEU

PHE

LEU

PRO

MET

16

SER

ALA

LYS

SER

Samples:

sample_one: basic fibroblast Growth Factor, [U-15N], 1 mM; DTT 10 mM; H2O 90%; D2O 10%; NaN3 2 mM; potassium phosphate 50 mM

sample_two: basic fibroblast Growth Factor, [U-15N; U-13C], 1 mM; DTT 10 mM; H2O 90%; D2O 10%; NaN3 2 mM; potassium phosphate 50 mM

sample_conditions_one: pH: 5.5; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions

Software:

No software information available

NMR spectrometers:

Bruker AM600 600 MHz

BMRB	18995
PDB	1BAS 1BFB 1BFC 1BFF 1BFG 1BLA 1BLD 1CVS 1EV2 1FGA 1FQ9 1II4 1IIL 2BFH 2FGF 2M49 4FGF 4OEE 4OEF 4OEG
DBJ	BAG70135 BAG70264
EMBL	CCO13911 CCO13913
GB	AAA31248 AAA52448 AAA52531 AAA52532 AAA52533
REF	NP_001103711 NP_001997 XP_001099284 XP_002815172 XP_003271404
SP	P09038 P48799 Q5IS69
AlphaFold	P09038 P48799 Q5IS69