BMRB Entry 4055

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4055

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Title:
Alpha-Helical Coiled Coil Trimerization Domain of Chicken Cartilage Matrix Protein (Oxidized)
Deposition date:
1997-08-07
Original release date:
1998-02-25
Authors:
Wiltscheck, Ronald; Kammerer, Richard; Dames, Sonja; Schulthess, Therese; Blommers, Marcel; Engel, Jurgen; Alexandrescu, Andrei
Citation:

Citation: Wiltscheck, Ronald; Kammerer, Richard; Dames, Sonja; Schulthess, Therese; Blommers, Marcel; Engel, Jurgen; Alexandrescu, Andrei. "Heteronuclear NMR Assignments and Secondary Structure of the Coiled Coil Trimerization Domain from Cartilage Matrix Protein in Oxidized and Reduced Forms."  Protein Sci. 6, 1734-1745 (1997).

Assembly members:

Assembly members:
Coiled Coil domain of Chicken Cartilage Matrix Protein, polymer, 47 residues, 5385 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Coiled Coil domain of Chicken Cartilage Matrix Protein: GSHMEEDPCECKSIVKFQTK VEELINTLQQKLEAVAKRIE ALENKII

Data sets:
Data typeCount
13C chemical shifts163
15N chemical shifts46
1H chemical shifts324

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1
2subunit B1
3subunit C1

Entities:

Entity 1, subunit A 47 residues - 5385 Da.

1   GLYSERHISMETGLUGLUASPPROCYSGLU
2   CYSLYSSERILEVALLYSPHEGLNTHRLYS
3   VALGLUGLULEUILEASNTHRLEUGLNGLN
4   LYSLEUGLUALAVALALALYSARGILEGLU
5   ALALEUGLUASNLYSILEILE

Samples:

sample_one: Coiled Coil domain of Chicken Cartilage Matrix Protein, [U-15N], 1 – 4 mM; NaCl 150 mM

sample_two: Coiled Coil domain of Chicken Cartilage Matrix Protein, [U-13C], 1 – 4 mM; NaCl 150 mM

sample_three: Coiled Coil domain of Chicken Cartilage Matrix Protein, [U-15N; U-13C], 1 – 4 mM; NaCl 150 mM

sample_four: Coiled Coil domain of Chicken Cartilage Matrix Protein, [U-15N; U-13C], 1 – 2 mM; Coiled Coil domain of Chicken Cartilage Matrix Protein, unlabeled, 1 – 2 mM; NaCl 150 mM

experimental_conditions_CMPcc_oxidized: pH: 5.7; pressure: 1 atm; temperature: 373 K

Experiments:

NameSampleSample stateSample conditions
not availablenot availablenot availableexperimental_conditions_CMPcc_oxidized

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz

Related Database Links:

BMRB 4054
PDB
EMBL CAA30915
GB AAA48695
REF NP_001025546
SP P05099
AlphaFold P05099

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