BMRB Entry 4054

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR4054

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Title:
Alpha-Helical Coiled Coil Trimerization Domain of Chicken Cartilage Matrix Protein (Reduced C5-C7 Disulfide Bonds)
Deposition date:
1997-08-07
Original release date:
1998-02-25
Authors:
Wiltscheck, Ronald; Kammerer, Richard; Dames, Sonja; Schulthess, Therese; Blommers, Marcel; Engel, Jurgen; Alexandrescu, Andrei
Citation:

Citation: Wiltscheck, Ronald; Kammerer, Richard; Dames, Sonja; Schulthess, Therese; Blommers, Marcel; Engel, Jurgen; Alexandrescu, Andrei. "Heteronuclear NMR Assignments and Secondary Structure of the Coiled Coil Trimerization Domain from Cartilage Matrix Protein in Oxidized and Reduced Forms."  Protein Sci. 6, 1734-1745 (1997).

Assembly members:

Assembly members:
Coiled Coil domain of Chicken Cartilage Matrix Protein, polymer, 47 residues, 5386 Da.

Natural source:

Natural source:   Common Name: chicken   Taxonomy ID: 9031   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Gallus gallus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET-15b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Coiled Coil domain of Chicken Cartilage Matrix Protein: GSHMEEDPCECKSIVKFQTK VEELINTLQQKLEAVAKRIE ALENKII

Data sets:
Data typeCount
15N chemical shifts40
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1
2subunit B1
3subunit C1

Entities:

Entity 1, subunit A 47 residues - 5386 Da.

1   GLYSERHISMETGLUGLUASPPROCYSGLU
2   CYSLYSSERILEVALLYSPHEGLNTHRLYS
3   VALGLUGLULEUILEASNTHRLEUGLNGLN
4   LYSLEUGLUALAVALALALYSARGILEGLU
5   ALALEUGLUASNLYSILEILE

Samples:

sample_CMPcc_reduced: Coiled Coil domain of Chicken Cartilage Matrix Protein, [U-15N], 1 mM; DTT 32 mM; NaCl 150 mM

experimental_conditions_CMPcc_reduced: pH: 5.8; pressure: 1 atm; temperature: 373 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_CMPcc_reducednot availableexperimental_conditions_CMPcc_reduced

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz

Related Database Links:

BMRB 4055
PDB
EMBL CAA30915
GB AAA48695
REF NP_001025546
SP P05099
AlphaFold P05099

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks