BMRB

Biological Magnetic Resonance Data Bank


A Repository for Data from NMR Spectroscopy on Proteins, Peptides, Nucleic Acids, and other Biomolecules
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BMRB Entry 4054

Title: Alpha-Helical Coiled Coil Trimerization Domain of Chicken Cartilage Matrix Protein (Reduced C5-C7 Disulfide Bonds)

Deposition date: 1997-08-07 Original release date: 1998-02-25

Authors: Wiltscheck, Ronald; Kammerer, Richard; Dames, Sonja; Schulthess, Therese; Blommers, Marcel; Engel, Jurgen; Alexandrescu, Andrei

Citation: Wiltscheck, Ronald; Kammerer, Richard; Dames, Sonja; Schulthess, Therese; Blommers, Marcel; Engel, Jurgen; Alexandrescu, Andrei. "Heteronuclear NMR Assignments and Secondary Structure of the Coiled Coil Trimerization Domain from Cartilage Matrix Protein in Oxidized and Reduced Forms."  Protein Sci. 6, 1734-1745 (1997).

Assembly members:
Coiled Coil domain of Chicken Cartilage Matrix Protein, polymer, 47 residues, 5386 Da.

Natural source:   Common Name: not available   Taxonomy ID: 9031   Superkingdom: not available   Kingdom: not available   Genus/species: not available not available

Experimental source:   Production method: recombinant technology' 'Escherichia coli' 'E. coli

Entity Sequences (FASTA):
Coiled Coil domain of Chicken Cartilage Matrix Protein: GSHMEEDPCECKSIVKFQTK VEELINTLQQKLEAVAKRIE ALENKII

Data sets:
Data typeCount
15N chemical shifts40
1H chemical shifts202

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1subunit A1
2subunit B1
3subunit C1

Entities:

Entity 1, subunit A 47 residues - 5386 Da.

1   GLYSERHISMETGLUGLUASPPROCYSGLU
2   CYSLYSSERILEVALLYSPHEGLNTHRLYS
3   VALGLUGLULEUILEASNTHRLEUGLNGLN
4   LYSLEUGLUALAVALALALYSARGILEGLU
5   ALALEUGLUASNLYSILEILE

Samples:

sample_CMPcc_reduced: Coiled Coil domain of Chicken Cartilage Matrix Protein, [U-15N], 1 mM; DTT 32 mM; NaCl 150 mM

experimental_conditions_CMPcc_reduced: pH: 5.8; pressure: 1 atm; temperature: 373 K

Experiments:

NameSampleSample stateSample conditions
not availablesample_CMPcc_reducednot availableexperimental_conditions_CMPcc_reduced

Software:

No software information available

NMR spectrometers:

  • Bruker AMX600 600 MHz

Related Database Links:

BMRB 4055
PDB
EMBL CAA30915
GB AAA48695
REF NP_001025546
SP P05099

Download simulated HSQC data in one of the following formats:
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