BMRB Entry 26891

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26891

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Title:
Solution and solid-state NMR studies of soluble tissue factor
Deposition date:
2016-09-08
Original release date:
2016-10-04
Authors:
Nuzzio, Kristin; Watt, Eric; Boettcher, John; Gajsiewicz, Joshua; Morrissey, James; Rienstra, Chad
Citation:

Citation: Nuzzio, Kristin; Watt, Eric; Boettcher, John; Gajsiewicz, Joshua; Morrissey, James; Rienstra, Chad. "High-Resolution NMR Studies of Human Tissue Factor"  PLOS ONE 11, e0163206-e0163206 (2016).
PubMed: 27657719

Assembly members:

Assembly members:
Extracellular_domain_of_tissue_factor, polymer, 229 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pJH677

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Extracellular_domain_of_tissue_factor: ATTNTVAAYNLTWKSTNFKT ILEWEPKPVNQVYTVQISTK SGDWKSKCFYTTDTECDLTD EIVKDVKQTYLARVFSYPAG NVESTGSAGEPLYENSPEFT PYLETNLGQPTIQSFEQVGT KVNVTVEDERTLVRRNNTFL SLRDVFGKDLIYTLYYWKSS SSGKKTAKTNTNEFLIDVDK GENYCFSVQAVIPSRTVNRK STDSPVECMGQEKGEFREGG AAGHHHHHH

Data typeCount

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1sTF monomer1

Entities:

Entity 1, sTF monomer 229 residues - Formula weight is not available

1   ALATHRTHRASNTHRVALALAALATYRASN
2   LEUTHRTRPLYSSERTHRASNPHELYSTHR
3   ILELEUGLUTRPGLUPROLYSPROVALASN
4   GLNVALTYRTHRVALGLNILESERTHRLYS
5   SERGLYASPTRPLYSSERLYSCYSPHETYR
6   THRTHRASPTHRGLUCYSASPLEUTHRASP
7   GLUILEVALLYSASPVALLYSGLNTHRTYR
8   LEUALAARGVALPHESERTYRPROALAGLY
9   ASNVALGLUSERTHRGLYSERALAGLYGLU
10   PROLEUTYRGLUASNSERPROGLUPHETHR
11   PROTYRLEUGLUTHRASNLEUGLYGLNPRO
12   THRILEGLNSERPHEGLUGLNVALGLYTHR
13   LYSVALASNVALTHRVALGLUASPGLUARG
14   THRLEUVALARGARGASNASNTHRPHELEU
15   SERLEUARGASPVALPHEGLYLYSASPLEU
16   ILETYRTHRLEUTYRTYRTRPLYSSERSER
17   SERSERGLYLYSLYSTHRALALYSTHRASN
18   THRASNGLUPHELEUILEASPVALASPLYS
19   GLYGLUASNTYRCYSPHESERVALGLNALA
20   VALILEPROSERARGTHRVALASNARGLYS
21   SERTHRASPSERPROVALGLUCYSMETGLY
22   GLNGLULYSGLYGLUPHEARGGLUGLYGLY
23   ALAALAGLYHISHISHISHISHISHIS

Samples:

sample_1: Extracellular domain of tissue factor, [U-15N], 100 uM; sodium phosphate 50 mM; sodium chloride 50 mM; sodium azide 0.01%; DSS 1 mM; D2O, [U-99% 2H], 10%

sample_2: Extracellular domain of tissue factor, [U-100% 13C; U-100% 15N], 45 mg/mL; Polyethylene Glycol 3350 20%; sodium phosphate 25 mM; sodium chloride 200 mM

sample_3: Extracellular domain of tissue factor, [U-100% 13C; U-100% 15N], 45 mg/mL; HEPES 100 mM; sodium chloride 200 mM; copper EDTA 5 mM; ammonium sulfate 1.8 M

sample_4: Extracellular domain of tissue factor, [U-100% 2-gly 13C; U-100% 15N; U-80% 2H], 45 mg/mL; HEPES 100 mM; sodium chloride 200 mM; copper EDTA 5 mM; ammonium sulfate 1.8 M

sample_conditions_1: ionic strength: 90 mM; pH: 6.5; pressure: 1 atm; temperature: 273 K

sample_conditions_2: pH: 7.5; temperature: 273 K

sample_conditions_3: pH: 7.5; temperature: 273 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 13C-13C DARRsample_2anisotropicsample_conditions_2
2D 13C-13C DARRsample_3anisotropicsample_conditions_3
2D 15N-13CAsample_3anisotropicsample_conditions_3
2D 15N-13CAsample_4anisotropicsample_conditions_3

Software:

VNMRJ, Varian - collection

SPARKY, Goddard - peak picking

NMR spectrometers:

  • Varian VNMRS 750 MHz
  • Varian VNMRS 500 MHz