BMRB Entry 26512

Name: Thermal stability of chicken brain a-spectrin repeat 17: a spectroscopic study
Published: 2015-02-25

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26512

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Thermal stability of chicken brain a-spectrin repeat 17: a spectroscopic study

Deposition date:

2015-02-17

Original release date:

2015-02-25

Authors:

Brenner, Annette; Kieffer, Bruno; Trave, Gilles; Froystein, Nils; Raae, Arnt

Citation:

Citation: Brenner, Annette; Kieffer, Bruno; Trave, Gilles; Froystein, Nils; Raae, Arnt. "Thermal stability of chicken brain a-spectrin repeat 17: a spectroscopic study" J. Biomol. NMR 53, 71-83 (2012).
PubMed: 22569754

Assembly members:

Assembly members:
a-spectrin, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: BL21(DE3)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
a-spectrin: MARGQRLEESLEYQQFVANV EEEEAWINEKMTLVASEDYG DTLAAIQGLLKKHEAFETDF TVHKDRVNDVCANGEDLIKK NNHHVENITAKMKGLKGKVS DLEKAAAQRKAKLDENSA

Data sets:

assigned_chemical_shifts
heteronucl_T1_relaxation
- heteronuclear_T1_list_1
heteronucl_T2_relaxation
- heteronuclear_T2_list_1

Data type	Count
13C chemical shifts	25
T1 relaxation values	65
T2 relaxation values	65

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	a-spectrin	1

Entities:

Entity 1, a-spectrin 118 residues - Formula weight is not available

1

MET

ALA

ARG

GLY

GLN

ARG

LEU

GLU

SER

2

LEU

GLU

TYR

GLN

PHE

VAL

ALA

ASN

VAL

3

GLU

ALA

TRP

ILE

ASN

GLU

LYS

4

MET

THR

LEU

VAL

ALA

SER

GLU

ASP

TYR

GLY

5

ASP

THR

LEU

ALA

ILE

GLN

GLY

LEU

6

LYS

HIS

GLU

ALA

PHE

GLU

THR

ASP

PHE

7

THR

VAL

HIS

LYS

ASP

ARG

VAL

ASN

ASP

VAL

8

CYS

ALA

ASN

GLY

GLU

ASP

LEU

ILE

LYS

9

ASN

HIS

VAL

GLU

ASN

ILE

THR

ALA

10

LYS

MET

LYS

GLY

LEU

LYS

GLY

LYS

VAL

SER

11

ASP

LEU

GLU

LYS

ALA

GLN

ARG

LYS

12

ALA

LYS

LEU

ASP

GLU

ASN

SER

ALA

Samples:

sample_1: a-spectrin, [U-100% 13C; U-100% 15N], 200 uM; D2O 10%; H2O 90%; Sodium phosphate buffer 20 mM; NaCl 0.1 M; DTT 1 mM; NaN3 0.05%

sample_conditions_1: temperature: 298 K; pH: 6.8; pressure: 1 atm

sample_conditions_2: temperature: 317 K; pH: 6.8; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

TOPSPIN, Bruker Biospin - processing

CARA, Keller and Wuthrich - resonance assignment

CCPNMR, CCPN - data analysis

NMR spectrometers:

Bruker Biospin AV600 600 MHz