BMRB Entry 18260

Name: 1H, 13C and 15N resonance assignment of chicken brain alpha spectrin repeat 17
Published: 2012-05-22

Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR18260

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N resonance assignment of chicken brain alpha spectrin repeat 17

Deposition date:

2012-02-14

Original release date:

2012-05-22

Authors:

Brenner, Annette; Raae, Arnt

Citation:

Citation: Brenner, Annette; Kieffer, Bruno; Trave, Gilles; Frystein, Nils Age; Raae, Arnt. "Thermal stability of chicken brain -spectrin repeat 17: a spectroscopic study." J. Biomol. NMR 53, 71-83 (2012).
PubMed: 22569754

Assembly members:

Assembly members:
R17, polymer, 118 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETM-12

Entity Sequences (FASTA):

Entity Sequences (FASTA):
R17: MARGQRLEESLEYQQFVANV EEEEAWINEKMTLVASEDYG DTLAAIQGLLKKHEAFETDF TVHKDRVNDVCANGEDLIKK NNHHVENITAKMKGLKGKVS DLEKAAAQRKAKLDENSA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
coupling_constants
- coupling_constant_list_1
heteronucl_NOEs
- heteronuclear_noe_list_1
heteronucl_T1_relaxation
- heteronuclear_T1_list_1
heteronucl_T2_relaxation
- heteronuclear_T2_list_1

Data type	Count
13C chemical shifts	483
15N chemical shifts	122
1H chemical shifts	770
T1 relaxation values	97
T2 relaxation values	97
coupling constants	94
heteronuclear NOE values	94

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	R17	1

Entities:

Entity 1, R17 118 residues - Formula weight is not available

Thew first residue is a remain of the TEV cleavage site

1

MET

ALA

ARG

GLY

GLN

ARG

LEU

GLU

SER

2

LEU

GLU

TYR

GLN

PHE

VAL

ALA

ASN

VAL

3

GLU

ALA

TRP

ILE

ASN

GLU

LYS

4

MET

THR

LEU

VAL

ALA

SER

GLU

ASP

TYR

GLY

5

ASP

THR

LEU

ALA

ILE

GLN

GLY

LEU

6

LYS

HIS

GLU

ALA

PHE

GLU

THR

ASP

PHE

7

THR

VAL

HIS

LYS

ASP

ARG

VAL

ASN

ASP

VAL

8

CYS

ALA

ASN

GLY

GLU

ASP

LEU

ILE

LYS

9

ASN

HIS

VAL

GLU

ASN

ILE

THR

ALA

10

LYS

MET

LYS

GLY

LEU

LYS

GLY

LYS

VAL

SER

11

ASP

LEU

GLU

LYS

ALA

GLN

ARG

LYS

12

ALA

LYS

LEU

ASP

GLU

ASN

SER

ALA

Samples:

sample_1: R17, [U-98% 13C; U-98% 15N], 0.2 ± 0.02 mM; sodium chloride 0.1 mM; DTT 0.05 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.1 M; pH: 6.8; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D C(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HBHA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D H(CCO)NH	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN v1.3, CCPN, Keller and Wuthrich - chemical shift assignment, peak picking

NMR spectrometers:

Bruker Avance 600 MHz

UNP	P07751
AlphaFold	P07751