BMRB Entry 26318

Name: Backbone 1H, 13C, 15N and CB Chemical Shift Assignments for RRM2/3
Published: 2022-05-11

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR26318

NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry

Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

Select model:

I am color blind

Download raw data. Direct link to this display. Help

Title:

Backbone 1H, 13C, 15N and CB Chemical Shift Assignments for RRM2/3

Deposition date:

2021-09-07

Original release date:

2022-05-11

Authors:

Sagae, Takeru; Yokogawa, Mariko; Osawa, Masanori

Citation:

Citation: Sagae, Takeru; Yokogawa, Mariko; Sawazaki, Ryoichi; Ishii, Yuichiro; Hosoda, Nao; Hoshino, Shin-ichi; Imai, Shunsuke; Shimada, Ichio; Osawa, Masanori. "Paip2 competitively dissociates PABPC1 from poly(A) by initial access to RRM2 of the poly(A)-bound PABPC1" J. Biol. Chem. 298, 101844-101844 (2022).
PubMed: 35307347

Assembly members:

Assembly members:
RRM2/3 of PABPC1, polymer, 195 residues, 22202 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-6p-1

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RRM2/3 of PABPC1: GPLGSNIFIKNLDKSIDNKA LYDTFSAFGNILSCKVVCDE NGSKGYGFVHFETQEAAERA IEKMNGMLLNDRKVFVGRFK SRKEREAELGARAKEFTNVY IKNFGEDMDDERLKDLFGKF GPALSVKVMTDESGKSKGFG FVSFERHEDAQKAVDEMNGK ELNGKQIYVGRAQKKVERQT ELKRKFEQMKQDRIT

Data sets:

assigned_chemical_shifts
- assigned_chemical_shifts_1

Data type	Count
13C chemical shifts	314
15N chemical shifts	182
1H chemical shifts	182

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	subunit 1	1

Entities:

Entity 1, subunit 1 195 residues - 22202 Da.

1

GLY

PRO

LEU

GLY

SER

ASN

ILE

PHE

ILE

LYS

2

ASN

LEU

ASP

LYS

SER

ILE

ASP

ASN

LYS

ALA

3

LEU

TYR

ASP

THR

PHE

SER

ALA

PHE

GLY

ASN

4

ILE

LEU

SER

CYS

LYS

VAL

CYS

ASP

GLU

5

ASN

GLY

SER

LYS

GLY

TYR

GLY

PHE

VAL

HIS

6

PHE

GLU

THR

GLN

GLU

ALA

GLU

ARG

ALA

7

ILE

GLU

LYS

MET

ASN

GLY

MET

LEU

ASN

8

ASP

ARG

LYS

VAL

PHE

VAL

GLY

ARG

PHE

LYS

9

SER

ARG

LYS

GLU

ARG

GLU

ALA

GLU

LEU

GLY

10

ALA

ARG

ALA

LYS

GLU

PHE

THR

ASN

VAL

TYR

11

ILE

LYS

ASN

PHE

GLY

GLU

ASP

MET

ASP

12

GLU

ARG

LEU

LYS

ASP

LEU

PHE

GLY

LYS

PHE

13

GLY

PRO

ALA

LEU

SER

VAL

LYS

VAL

MET

THR

14

ASP

GLU

SER

GLY

LYS

SER

LYS

GLY

PHE

GLY

15

PHE

VAL

SER

PHE

GLU

ARG

HIS

GLU

ASP

ALA

16

GLN

LYS

ALA

VAL

ASP

GLU

MET

ASN

GLY

LYS

17

GLU

LEU

ASN

GLY

LYS

GLN

ILE

TYR

VAL

GLY

18

ARG

ALA

GLN

LYS

VAL

GLU

ARG

GLN

THR

19

GLU

LEU

LYS

ARG

LYS

PHE

GLU

GLN

MET

LYS

20

GLN

ASP

ARG

ILE

THR

Samples:

sample_1: RRM2/3 of PABPC1, [U-100% 13C; U-100% 15N], 507 uM; sodium phosphate 18 mM; sodium chloride 135 mM; H2O 90%; D2O, [U-2H], 10%; DTT 0.9 mM

sample_conditions_1: pH: 6.5; pressure: 1 atm; temperature: 303 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1

Software:

SPARKY - chemical shift assignment

NMR spectrometers:

Bruker AVANCE NEO 600 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks