BMRB Entry 25676

Title:
Backbone resonance assignments of the mutant NS4A N-terminal domain
Deposition date:
2015-06-30
Original release date:
2015-07-24
Authors:
Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd
Citation:

Citation: Hung, Yu-Fu; Schwarten, Melanie; Hoffmann, Silke; Willbold, Dieter; Sklan, Ella; Koenig, Bernd. "Amino Terminal Region of Dengue Virus NS4A Cytosolic Domain Binds to Highly Curved Liposomes."  Viruses 7, 4119-4130 (2015).
PubMed: 26197333

Assembly members:

Assembly members:
molecule_1, polymer, 48 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Aeropyrum pernix   Taxonomy ID: 56636   Superkingdom: Archaea   Kingdom: not available   Genus/species: Aeropyrum pernix

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
molecule_1: SLTLNEITEEGRLPTFMTQK ARDALDNLAVLHTAEAGGRA YNHALSEL

Data sets:
Data typeCount
13C chemical shifts134
15N chemical shifts41
1H chemical shifts41

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1NS4A mut1

Entities:

Entity 1, NS4A mut 48 residues - Formula weight is not available

1   SERLEUTHRLEUASNGLUILETHRGLUGLU
2   GLYARGLEUPROTHRPHEMETTHRGLNLYS
3   ALAARGASPALALEUASPASNLEUALAVAL
4   LEUHISTHRALAGLUALAGLYGLYARGALA
5   TYRASNHISALALEUSERGLULEU

Samples:

NMR_sample: mutNS4A, [U-13C; U-15N], 200 uM; Sodium Phosphate 50 mM; Sodium Azide 0.03%; Sodium Chloride 150 mM; H2O 90%; D2O, [U-2H], 10%

sample_conditions_1: ionic strength: 200 mM; pH: 6.8; pressure: 1.0 atm; temperature: 303.0 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCNMR_sampleisotropicsample_conditions_1
3D HNCANMR_sampleisotropicsample_conditions_1
3D HNCACONMR_sampleisotropicsample_conditions_1
3D HNCACBNMR_sampleisotropicsample_conditions_1
3D HNCONMR_sampleisotropicsample_conditions_1

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMR spectrometers:

  • Bruker Avance III HD 700 MHz

Related Database Links:

BMRB 25180

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks