BMRB Entry 25180

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25180

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Title:
Backbone resonance assignments of the mutant NS4A N-terminal domain of DENV (L6E;M10E)
Deposition date:
2014-08-29
Original release date:
2015-03-25
Authors:
Willbold, Dieter; Koenig, Bernd; Hung, Yu-Fu; Schwarten, Melanie
Citation:

Citation: Hung, Yu-Fu; Schwarten, Melanie; Schuenke, Sven; Thiagarajan, Pallavi; Koenig, Bernd; Hoffmann, Silke; Sklan, Ella; Willbold, Dieter. "Dengue virus NS4A cytoplasmic domain binding to liposomes is sensitive to membrane curvature"  Biochim. Biophys. Acta. 1848, 1119-1126 (2015).
PubMed: 25644870

Assembly members:

Assembly members:
mutNS4A, polymer, 48 residues, 5238.8267 Da.

Natural source:

Natural source:   Common Name: DENV Type 2   Taxonomy ID: 11060   Superkingdom: Viruses   Kingdom: not available   Genus/species: Flavivirus Dengue virus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGEX-4T-2

Entity Sequences (FASTA):

Entity Sequences (FASTA):
mutNS4A: SLTLNEITEEGRLPTFMTQK ARDALDNLAVLHTAEAGGRA YNHALSEL

Data sets:
Data typeCount
1H chemical shifts159
13C chemical shifts137
15N chemical shifts46

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1mutNS4A1

Entities:

Entity 1, mutNS4A 48 residues - 5238.8267 Da.

1   SERLEUTHRLEUASNGLUILETHRGLUGLU
2   GLYARGLEUPROTHRPHEMETTHRGLNLYS
3   ALAARGASPALALEUASPASNLEUALAVAL
4   LEUHISTHRALAGLUALAGLYGLYARGALA
5   TYRASNHISALALEUSERGLULEU

Samples:

NMR_sample: mutNS4A, [U-13C; U-15N], 1.0 mM; Sodium Dodecyl Sulfate, [U-2H], 100.0 mM; Sodium Phosphate 50.0 mM; sodium azide 0.03 % w/v; H2O 90%; D2O 10%

NMR_sample_ref: mutNS4A, [U-13C; U-15N], 1.0 mM; Sodium Dodecyl Sulfate, [U-2H], 100.0 mM; Sodium Phosphate 50.0 mM; DSS 10 mM; sodium azide 0.03 % w/v; H2O 90%; D2O 10%

Set_1: ionic strength: 150.000 mM; pH: 6.800; pressure: 1.000 atm; temperature: 303.000 K

Set_2: ionic strength: 150.000 mM; pH: 6.800; pressure: 1.000 atm; temperature: 303.000 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQC/HMQCNMR_sampleisotropicSet_1
3D HNCONMR_sampleisotropicSet_1
3D HNCACBNMR_sampleisotropicSet_1
HNHA (H[N[ca[HA]]])NMR_sampleisotropicSet_1
2D 1H-13C HSQC/HMQCNMR_sample_refisotropicSet_2

Software:

CcpNmr_Analysis v2.4, CCPN - chemical shift assignment

NMRPipe v8.1, NIH - processing

TALOS-N v4.01, Yang Shen and Ad Bax - data analysis

NMR spectrometers:

  • Varian VNMRS 800 MHz
  • Varian Unity_INOVA 600 MHz

Related Database Links:

GenBank np739588
BMRB 25676

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks