BMRB Entry 25433

Name: NMR structure of the first Zinc Finger domain of RBM10
Published: 2015-01-26

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PDB ID: 2mxv
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR25433
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

NMR structure of the first Zinc Finger domain of RBM10

Deposition date:

2015-01-16

Original release date:

2015-01-26

Authors:

Serrano, Pedro; Wuthrich, Kurt; Geralt, Michael

Citation:

Citation: Serrano, Pedro; Geralt, Michael; Wuthrich, Kurt. "NMR structure of the first Zinc Finger domain of RBM10" .

Assembly members:

Assembly members:
entity_1, polymer, 43 residues, 4957.872 Da.
ZINC ION, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET28b

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: GHMPKINEDWLCNKCGVQNF KRREKCFKCGVPKSEAEQKL PLG

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	228
13C chemical shifts	140
15N chemical shifts	39

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	entity_1	1
2	ZINC ION	2

Entities:

Entity 1, entity_1 43 residues - 4957.872 Da.

1

GLY

HIS

MET

PRO

LYS

ILE

ASN

GLU

ASP

TRP

2

LEU

CYS

ASN

LYS

CYS

GLY

VAL

GLN

ASN

PHE

3

LYS

ARG

GLU

LYS

CYS

PHE

LYS

CYS

GLY

4

VAL

PRO

LYS

SER

GLU

ALA

GLU

GLN

LYS

LEU

5

PRO

LEU

GLY

Entity 2, ZINC ION - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: entity, [U-99% 13C; U-98% 15N], 1.0 mM; sodium phosphate 20 mM; sodium chloride 50 mM; sodium azide 5 mM; H2O 95%; D2O 5%

sample_conditions_1: temperature: 298 K; pH: 6.0; pressure: 1 atm; ionic strength: 0.0798 M

Experiments:

Name	Sample	Sample state	Sample conditions
APSY 4D-HACANH	sample_1	isotropic	sample_conditions_1
APSY 5D-HACACONH	sample_1	isotropic	sample_conditions_1
APSY 5D-CBCACONH	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aromatic	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

CNS, Brunger A. T. et.al., Herrman & Wuthrich - refinement, chemical shift assignment, peak picking, structure solution

CYANA, Guntert, Mumenthaler and Wuthrich - structure solution

CARA, Keller and Wuthrich - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

BMRB	17386
PDB	2MXV
DBJ	BAA09471 BAA12144 BAC31087 BAC40753 BAC65490
EMBL	CAD97933 CAL37951
GB	AAH00681 AAH03089 AAH04181 AAH04674 AAH08733
REF	NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397
SP	P70501 P98175 Q99KG3
TPG	DAA12902 DAA12903
AlphaFold	P70501 P98175 Q99KG3