BMRB Entry 17386

Name: RBM10 RanBP2 zinc finger backbone chemical shifts
Published: 2011-01-28

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR17386

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

RBM10 RanBP2 zinc finger backbone chemical shifts

Deposition date:

2010-12-23

Original release date:

2011-01-28

Authors:

Mansfield, Robyn; Mackay, Joel; Nguyen, Cuong

Citation:

Citation: Nguyen, Cuong; Mansfield, Robyn; Leung, Wilfred; Vaz, Paula; Loughlin, Fionna; Grant, Richard; Mackay, Joel. "Characterization of a Family of RanBP2-Type Zinc Fingers that Can Recognize Single-Stranded RNA." J. Mol. Biol. 407, 273-283 (2011).
PubMed: 21256132

Assembly members:

Assembly members:
RBM10_ZnF, polymer, 41 residues, Formula weight is not available
ZN, non-polymer, 65.409 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pGEX-2T

Entity Sequences (FASTA):

Entity Sequences (FASTA):
RBM10_ZnF: GSSDPKPKINEDWLCNKCGV QNFKRREKCFKCGVPKSEAE Q

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	76
15N chemical shifts	33
1H chemical shifts	33

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	protein component	1
2	zinc ion	2

Entities:

Entity 1, protein component 41 residues - Formula weight is not available

N-terminal GS is non-native, resulting from cleavage of a GST tag.

1

GLY

SER

ASP

PRO

LYS

PRO

LYS

ILE

ASN

2

GLU

ASP

TRP

LEU

CYS

ASN

LYS

CYS

GLY

VAL

3

GLN

ASN

PHE

LYS

ARG

GLU

LYS

CYS

PHE

4

LYS

CYS

GLY

VAL

PRO

LYS

SER

GLU

ALA

GLU

5

GLN

Entity 2, zinc ion - Zn - 65.409 Da.

1

ZN

Samples:

sample_1: RBM10 ZnF, [U-100% 13C; U-100% 15N], 1 mM; DTT 1 mM; sodium phosphate 25 mM; sodium chloride 100 mM; DSS 20 uM; H2O 93%; D2O 7%

sample_conditions_1: ionic strength: 0.125 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Bruker DRX 600 MHz

BMRB	25433
PDB	2MXV
DBJ	BAA09471 BAA12144 BAC31087 BAC40753 BAC65490
EMBL	CAD97933 CAL37951
GB	AAH00681 AAH03089 AAH04181 AAH04674 AAH08733
REF	NP_001161247 NP_001161248 NP_001191395 NP_001191396 NP_001191397
SP	P70501 P98175 Q99KG3
TPG	DAA12902 DAA12903
AlphaFold	P70501 P98175 Q99KG3