BMRB Entry 25290
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25290
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Citation: Ward, Meaghan; Wang, Shenlin; Munro, Rachel; Ritz, Emily; Hung, Ivan; Gor'kov, Peter; Jiang, Yunjiang; Liang, Hongjun; Brown, Leonid; Ladizhansky, Vladimir. "In Situ Structural Studies of Anabaena Sensory Rhodopsin in the E. coli Membrane" Biophys. J. 108, 1683-1696 (2015).
Assembly members:
Anabaena_sensory_rhodopsin, polymer, 235 residues, Formula weight is not available
Natural source: Common Name: Anabaena (Nostoc) Taxonomy ID: 103690 Superkingdom: Bacteria Kingdom: not available Genus/species: Anabaena PCC7120
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pKJ606
Entity Sequences (FASTA):
Anabaena_sensory_rhodopsin: MNLESLLHWIYVAGMTIGAL
HFWSLSRNPRGVPQYEYLVA
MFIPIWSGLAYMAMAIDQGK
VEAAGQIAHYARYIDWMVTT
PLLLLSLSWTAMQFIKKDWT
LIGFLMSTQIVVITSGLIAD
LSERDWVRYLWYICGVCAFL
IILWGIWNPLRAKTRTQSSE
LANLYDKLVTYFTVLWIGYP
IVWIIGPSGFGWINQTIDTF
LFCLLPFFSKVGFSFLDLHG
LRNLNDSRQHHHHHH
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 167 |
| 15N chemical shifts | 82 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | ASR, chain 1 | 1 |
| 2 | ASR, chain 2 | 1 |
| 3 | ASR, chain 3 | 1 |
Entities:
Entity 1, ASR, chain 1 235 residues - Formula weight is not available
| 1 | MET | ASN | LEU | GLU | SER | LEU | LEU | HIS | TRP | ILE | ||||
| 2 | TYR | VAL | ALA | GLY | MET | THR | ILE | GLY | ALA | LEU | ||||
| 3 | HIS | PHE | TRP | SER | LEU | SER | ARG | ASN | PRO | ARG | ||||
| 4 | GLY | VAL | PRO | GLN | TYR | GLU | TYR | LEU | VAL | ALA | ||||
| 5 | MET | PHE | ILE | PRO | ILE | TRP | SER | GLY | LEU | ALA | ||||
| 6 | TYR | MET | ALA | MET | ALA | ILE | ASP | GLN | GLY | LYS | ||||
| 7 | VAL | GLU | ALA | ALA | GLY | GLN | ILE | ALA | HIS | TYR | ||||
| 8 | ALA | ARG | TYR | ILE | ASP | TRP | MET | VAL | THR | THR | ||||
| 9 | PRO | LEU | LEU | LEU | LEU | SER | LEU | SER | TRP | THR | ||||
| 10 | ALA | MET | GLN | PHE | ILE | LYS | LYS | ASP | TRP | THR | ||||
| 11 | LEU | ILE | GLY | PHE | LEU | MET | SER | THR | GLN | ILE | ||||
| 12 | VAL | VAL | ILE | THR | SER | GLY | LEU | ILE | ALA | ASP | ||||
| 13 | LEU | SER | GLU | ARG | ASP | TRP | VAL | ARG | TYR | LEU | ||||
| 14 | TRP | TYR | ILE | CYS | GLY | VAL | CYS | ALA | PHE | LEU | ||||
| 15 | ILE | ILE | LEU | TRP | GLY | ILE | TRP | ASN | PRO | LEU | ||||
| 16 | ARG | ALA | LYS | THR | ARG | THR | GLN | SER | SER | GLU | ||||
| 17 | LEU | ALA | ASN | LEU | TYR | ASP | LYS | LEU | VAL | THR | ||||
| 18 | TYR | PHE | THR | VAL | LEU | TRP | ILE | GLY | TYR | PRO | ||||
| 19 | ILE | VAL | TRP | ILE | ILE | GLY | PRO | SER | GLY | PHE | ||||
| 20 | GLY | TRP | ILE | ASN | GLN | THR | ILE | ASP | THR | PHE | ||||
| 21 | LEU | PHE | CYS | LEU | LEU | PRO | PHE | PHE | SER | LYS | ||||
| 22 | VAL | GLY | PHE | SER | PHE | LEU | ASP | LEU | HIS | GLY | ||||
| 23 | LEU | ARG | ASN | LEU | ASN | ASP | SER | ARG | GLN | HIS | ||||
| 24 | HIS | HIS | HIS | HIS | HIS |
Samples:
IM-ASR: IM-ASR, [U-100% 13C; U-100% 15N], 0.053 mg/ul; E. coli inner membrane 0.93 mg/ul; H2O 100%
sample_conditions_1: pH: 9; pressure: 1 atm; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D NCA | IM-ASR | solid | sample_conditions_1 |
| 3D CANCO | IM-ASR | solid | sample_conditions_1 |
| 3D NCACB | IM-ASR | solid | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
CARA, Keller and Wuthrich - data analysis
NMR spectrometers:
- Bruker Avance 800 MHz
- Bruker Avance 900 MHz