BMRB Entry 17064
Chem Shift validation: AVS_anomalous, AVS_full
BMRB Entry DOI: doi:10.13018/BMR17064
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Citation: Shi, Lichi; Kawamura, Izuru; Jung, Kwang-Hwan; Brown, Leonid; Ladizhansky, Vladimir. "Conformation of a Seven-Helical Transmembrane Photosensor in the Lipid Environment." Angew. Chem. Int. Ed. Engl. ., .-. (2010).
PubMed: 21191921
Assembly members:
opsin, polymer, 235 residues, 27220 Da.
RET, non-polymer, 284.436 Da.
Natural source: Common Name: Anabaena PCC7120 Taxonomy ID: 103690 Superkingdom: Bacteria Kingdom: not available Genus/species: Nostoc not available
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: na
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 668 |
| 15N chemical shifts | 177 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | opsin | 1 |
| 2 | retinal | 2 |
Entities:
Entity 1, opsin 235 residues - 27220 Da.
| 1 | MET | ASN | LEU | GLU | SER | LEU | LEU | HIS | TRP | ILE | ||||
| 2 | TYR | VAL | ALA | GLY | MET | THR | ILE | GLY | ALA | LEU | ||||
| 3 | HIS | PHE | TRP | SER | LEU | SER | ARG | ASN | PRO | ARG | ||||
| 4 | GLY | VAL | PRO | GLN | TYR | GLU | TYR | LEU | VAL | ALA | ||||
| 5 | MET | PHE | ILE | PRO | ILE | TRP | SER | GLY | LEU | ALA | ||||
| 6 | TYR | MET | ALA | MET | ALA | ILE | ASP | GLN | GLY | LYS | ||||
| 7 | VAL | GLU | ALA | ALA | GLY | GLN | ILE | ALA | HIS | TYR | ||||
| 8 | ALA | ARG | TYR | ILE | ASP | TRP | MET | VAL | THR | THR | ||||
| 9 | PRO | LEU | LEU | LEU | LEU | SER | LEU | SER | TRP | THR | ||||
| 10 | ALA | MET | GLN | PHE | ILE | LYS | LYS | ASP | TRP | THR | ||||
| 11 | LEU | ILE | GLY | PHE | LEU | MET | SER | THR | GLN | ILE | ||||
| 12 | VAL | VAL | ILE | THR | SER | GLY | LEU | ILE | ALA | ASP | ||||
| 13 | LEU | SER | GLU | ARG | ASP | TRP | VAL | ARG | TYR | LEU | ||||
| 14 | TRP | TYR | ILE | CYS | GLY | VAL | CYS | ALA | PHE | LEU | ||||
| 15 | ILE | ILE | LEU | TRP | GLY | ILE | TRP | ASN | PRO | LEU | ||||
| 16 | ARG | ALA | LYS | THR | ARG | THR | GLN | SER | SER | GLU | ||||
| 17 | LEU | ALA | ASN | LEU | TYR | ASP | LYS | LEU | VAL | THR | ||||
| 18 | TYR | PHE | THR | VAL | LEU | TRP | ILE | GLY | TYR | PRO | ||||
| 19 | ILE | VAL | TRP | ILE | ILE | GLY | PRO | SER | GLY | PHE | ||||
| 20 | GLY | TRP | ILE | ASN | GLN | THR | ILE | ASP | THR | PHE | ||||
| 21 | LEU | PHE | CYS | LEU | LEU | PRO | PHE | PHE | SER | LYS | ||||
| 22 | VAL | GLY | PHE | SER | PHE | LEU | ASP | LEU | HIS | GLY | ||||
| 23 | LEU | ARG | ASN | LEU | ASN | ASP | SER | ARG | GLN | HIS | ||||
| 24 | HIS | HIS | HIS | HIS | HIS |
Entity 2, retinal - C20 H28 O - 284.436 Da.
| 1 | RET |
Samples:
sample_1: opsin, [U-99% 13C; U-99% 15N], 11 ± 1 mg; retinal 110 ± 10 ug; H2O 100%; NaCl 10 mM; CHES 25 mM
sample_conditions_1: ionic strength: 90 mM; pH: 9; pressure: 1 atm; temperature: 273 K
sample_conditions_2: ionic strength: 90 mM; pD: 9; temperature: 273 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 3D CONCA | sample_1 | solid | sample_conditions_1 |
| 3D NCACX | sample_1 | solid | sample_conditions_1 |
| 3D NCOCX | sample_1 | solid | sample_conditions_1 |
Software:
TOPSPIN, Bruker Biospin - collection
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
NMR spectrometers:
- Bruker Avance 800 MHz