BMRB Entry 25267

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25267

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Title:
Backbone 1H, 13C and 15N Chemical Shift Assignments for Human TRIM25 (PRYSPRY) domain
Deposition date:
2014-10-06
Original release date:
2015-09-04
Authors:
Yu, Chin; Penumutchu, Srinivasa
Citation:

Citation: Kong, Chen; Penumutchu, Srinivasa; Hung, Kuo-wei; Huang, Huiying; Lin, Tianwei; Yu, Chin. "Backbone resonance assignments of the PRYSPRY domain of TRIM25"  Biomol. NMR Assign. 9, 313-315 (2015).
PubMed: 25702035

Assembly members:

Assembly members:
TRIM25_(PRYSPRY)_domain, polymer, 202 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
TRIM25_(PRYSPRY)_domain: MSTSLKAKVLETFLAKSRPE LLEYYIKVILDYNTAHNKVA LSECYTVASVAEMPQNYRPH PQRFTYCSQVLGLHCYKKGI HYWEVELQKNNFCGVGICYG SMNRQGPESRLGRNSASWCV EWFNTKISAWHNNVEKTLPS TKATRVGVLLNCDHGFVIFF AVADKVHLMYKFRVDFTEAL YPAFWVFSAGATLSICSPKL EH

Data sets:
Data typeCount
13C chemical shifts544
15N chemical shifts183
1H chemical shifts183

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1TRIM25 (PRYSPRY) domain1

Entities:

Entity 1, TRIM25 (PRYSPRY) domain 202 residues - Formula weight is not available

1   METSERTHRSERLEULYSALALYSVALLEU
2   GLUTHRPHELEUALALYSSERARGPROGLU
3   LEULEUGLUTYRTYRILELYSVALILELEU
4   ASPTYRASNTHRALAHISASNLYSVALALA
5   LEUSERGLUCYSTYRTHRVALALASERVAL
6   ALAGLUMETPROGLNASNTYRARGPROHIS
7   PROGLNARGPHETHRTYRCYSSERGLNVAL
8   LEUGLYLEUHISCYSTYRLYSLYSGLYILE
9   HISTYRTRPGLUVALGLULEUGLNLYSASN
10   ASNPHECYSGLYVALGLYILECYSTYRGLY
11   SERMETASNARGGLNGLYPROGLUSERARG
12   LEUGLYARGASNSERALASERTRPCYSVAL
13   GLUTRPPHEASNTHRLYSILESERALATRP
14   HISASNASNVALGLULYSTHRLEUPROSER
15   THRLYSALATHRARGVALGLYVALLEULEU
16   ASNCYSASPHISGLYPHEVALILEPHEPHE
17   ALAVALALAASPLYSVALHISLEUMETTYR
18   LYSPHEARGVALASPPHETHRGLUALALEU
19   TYRPROALAPHETRPVALPHESERALAGLY
20   ALATHRLEUSERILECYSSERPROLYSLEU
21   GLUHIS

Samples:

sample_1: TRIM25 (PRYSPRY) domain, [U-99% 13C; U-99% 15N], 0.6 mM; Buffer: 20 mM Tris/HCl 20 mM; NaCl 100 mM; Arg 50 mM; Glue 50 mM; DTT 7 mM; D2O 10%; H2O 90%

sample_conditions_1: ionic strength: 0.1 M; pH: 7.3; pressure: 1 atm; temperature: 308 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(COCA)CBsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
2D 1H-15N HSQC NH2 onlysample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1

Software:

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

  • Varian INOVA 700 MHz

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks