BMRB Entry 25072
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25072
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
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Citation: Hennig, Janosch; Militti, Cristina; Popowicz, Gzegorz; Wang, Iren; Sonntag, Miriam; Geerlof, Arie; Gabel, Frank; Gebauer, Fatima; Sattler, Michael. "Structural basis for the assembly of the Sxl-Unr translation regulatory complex" Nature 515, 287-290 (2014).
PubMed: 25209665
Assembly members:
Sex-lethal_(SXL), polymer, 176 residues, Formula weight is not available
CSD1, polymer, 72 residues, Formula weight is not available
msl2-mRNA, polymer, 18 residues, Formula weight is not available
Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTRX-SXL
Entity Sequences (FASTA):
Sex-lethal_(SXL): GAMASNTNLIVNYLPQDMTD
RELYALFRAIGPINTCRIMR
DYKTGYSFGYAFVDFTSEMD
SQRAIKVLNGITVRNKRLKV
SYARPGGESIKDTNLYVTNL
PRTITDDQLDTIFGKYGSIV
QKNILRDKLTGRPRGVAFVR
YNKREEAQEAISALNNVIPE
GGSQPLSVRLAEEHGK
CSD1: GAMATRETGIIEKLLHSYGF
IQCCERQARLFFHFSQFSGN
IDHLKIGDPVEFEMTYDRRT
GKPIASQVSKIA
msl2-mRNA: UUUUUUUGAGCACGUGAA
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 152 |
| 13C chemical shifts | 130 |
| 15N chemical shifts | 152 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | Sex-lethal (SXL) | 1 |
| 2 | CSD1 (UNR) | 2 |
| 3 | msl2 mRNA | 3 |
Entities:
Entity 1, Sex-lethal (SXL) 176 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | SER | ASN | THR | ASN | LEU | ILE | ||||
| 2 | VAL | ASN | TYR | LEU | PRO | GLN | ASP | MET | THR | ASP | ||||
| 3 | ARG | GLU | LEU | TYR | ALA | LEU | PHE | ARG | ALA | ILE | ||||
| 4 | GLY | PRO | ILE | ASN | THR | CYS | ARG | ILE | MET | ARG | ||||
| 5 | ASP | TYR | LYS | THR | GLY | TYR | SER | PHE | GLY | TYR | ||||
| 6 | ALA | PHE | VAL | ASP | PHE | THR | SER | GLU | MET | ASP | ||||
| 7 | SER | GLN | ARG | ALA | ILE | LYS | VAL | LEU | ASN | GLY | ||||
| 8 | ILE | THR | VAL | ARG | ASN | LYS | ARG | LEU | LYS | VAL | ||||
| 9 | SER | TYR | ALA | ARG | PRO | GLY | GLY | GLU | SER | ILE | ||||
| 10 | LYS | ASP | THR | ASN | LEU | TYR | VAL | THR | ASN | LEU | ||||
| 11 | PRO | ARG | THR | ILE | THR | ASP | ASP | GLN | LEU | ASP | ||||
| 12 | THR | ILE | PHE | GLY | LYS | TYR | GLY | SER | ILE | VAL | ||||
| 13 | GLN | LYS | ASN | ILE | LEU | ARG | ASP | LYS | LEU | THR | ||||
| 14 | GLY | ARG | PRO | ARG | GLY | VAL | ALA | PHE | VAL | ARG | ||||
| 15 | TYR | ASN | LYS | ARG | GLU | GLU | ALA | GLN | GLU | ALA | ||||
| 16 | ILE | SER | ALA | LEU | ASN | ASN | VAL | ILE | PRO | GLU | ||||
| 17 | GLY | GLY | SER | GLN | PRO | LEU | SER | VAL | ARG | LEU | ||||
| 18 | ALA | GLU | GLU | HIS | GLY | LYS |
Entity 2, CSD1 (UNR) 72 residues - Formula weight is not available
| 1 | GLY | ALA | MET | ALA | THR | ARG | GLU | THR | GLY | ILE | ||||
| 2 | ILE | GLU | LYS | LEU | LEU | HIS | SER | TYR | GLY | PHE | ||||
| 3 | ILE | GLN | CYS | CYS | GLU | ARG | GLN | ALA | ARG | LEU | ||||
| 4 | PHE | PHE | HIS | PHE | SER | GLN | PHE | SER | GLY | ASN | ||||
| 5 | ILE | ASP | HIS | LEU | LYS | ILE | GLY | ASP | PRO | VAL | ||||
| 6 | GLU | PHE | GLU | MET | THR | TYR | ASP | ARG | ARG | THR | ||||
| 7 | GLY | LYS | PRO | ILE | ALA | SER | GLN | VAL | SER | LYS | ||||
| 8 | ILE | ALA |
Entity 3, msl2 mRNA 18 residues - Formula weight is not available
| 1 | U | U | U | U | U | U | U | G | A | G | ||||
| 2 | C | A | C | G | U | G | A | A |
Samples:
sample_1: Sex-lethal (SXL), [U-13C; U-15N; U-2H], 0.2 – 0.5 mM; CSD10.2 – 0.5 mM; msl20.2 – 0.5 mM; DTT 10 mM; potassium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02%
sample_conditions_1: temperature: 310 K; pH: 6.0; pressure: 1 atm
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D TROSY-HNCA | sample_1 | isotropic | sample_conditions_1 |
Software:
CARA, Fred Damberger - chemical shift assignment
NMR spectrometers:
- Bruker Avance 700 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks