BMRB Entry 25072

Title:
Sex-lethal in complex with Upstream-to-N-Ras and msl2 mRNA   PubMed: 25209665
Deposition date:
2014-07-03
Original release date:
2014-11-07
Authors:
Hennig, Janosch; Sattler, Michael
Citation:

Citation: Hennig, Janosch; Militti, Cristina; Popowicz, Gzegorz; Wang, Iren; Sonntag, Miriam; Geerlof, Arie; Gabel, Frank; Gebauer, Fatima; Sattler, Michael. "Structural basis for the assembly of the Sxl-Unr translation regulatory complex"  Nature 515, 287-290 (2014).

Assembly members:

Assembly members:
Sex-lethal_(SXL), polymer, 176 residues, Formula weight is not available
CSD1, polymer, 72 residues, Formula weight is not available
msl2-mRNA, polymer, 18 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTRX-SXL

Experimental source:

Natural source:   Common Name: fruit fly   Taxonomy ID: 7227   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Drosophila melanogaster

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pTRX-SXL

Data sets:
Data typeCount
1H chemical shifts152
13C chemical shifts130
15N chemical shifts152

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1Sex-lethal (SXL)1
2CSD1 (UNR)2
3msl2 mRNA3

Entities:

Entity 1, Sex-lethal (SXL) 176 residues - Formula weight is not available

1   GLYALAMETALASERASNTHRASNLEUILE
2   VALASNTYRLEUPROGLNASPMETTHRASP
3   ARGGLULEUTYRALALEUPHEARGALAILE
4   GLYPROILEASNTHRCYSARGILEMETARG
5   ASPTYRLYSTHRGLYTYRSERPHEGLYTYR
6   ALAPHEVALASPPHETHRSERGLUMETASP
7   SERGLNARGALAILELYSVALLEUASNGLY
8   ILETHRVALARGASNLYSARGLEULYSVAL
9   SERTYRALAARGPROGLYGLYGLUSERILE
10   LYSASPTHRASNLEUTYRVALTHRASNLEU
11   PROARGTHRILETHRASPASPGLNLEUASP
12   THRILEPHEGLYLYSTYRGLYSERILEVAL
13   GLNLYSASNILELEUARGASPLYSLEUTHR
14   GLYARGPROARGGLYVALALAPHEVALARG
15   TYRASNLYSARGGLUGLUALAGLNGLUALA
16   ILESERALALEUASNASNVALILEPROGLU
17   GLYGLYSERGLNPROLEUSERVALARGLEU
18   ALAGLUGLUHISGLYLYS

Entity 2, CSD1 (UNR) 72 residues - Formula weight is not available

1   GLYALAMETALATHRARGGLUTHRGLYILE
2   ILEGLULYSLEULEUHISSERTYRGLYPHE
3   ILEGLNCYSCYSGLUARGGLNALAARGLEU
4   PHEPHEHISPHESERGLNPHESERGLYASN
5   ILEASPHISLEULYSILEGLYASPPROVAL
6   GLUPHEGLUMETTHRTYRASPARGARGTHR
7   GLYLYSPROILEALASERGLNVALSERLYS
8   ILEALA

Entity 3, msl2 mRNA 18 residues - Formula weight is not available

1   UUUUUUUGAG
2   CACGUGAA

Samples:

sample_1: Sex-lethal (SXL), [U-13C; U-15N; U-2H], 0.2 – 0.5 mM; CSD10.2 – 0.5 mM; msl20.2 – 0.5 mM; DTT 10 mM; potassium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02%

sample_conditions_1: temperature: 310 K; pH: 6.0; pressure: 1 atm

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D TROSY-HNCAsample_1isotropicsample_conditions_1

Software:

CARA, Fred Damberger - chemical shift assignment

NMR spectrometers:

  • Bruker Avance 700 MHz

Related Database Links:

PDB
GB EDX02402
BMRB 25059 25060 25078

Download simulated HSQC data in one of the following formats:
CSV: Backbone or all simulated shifts
SPARKY: Backbone or all simulated shifts