BMRB Entry 25078

Name: CSD1-UNR bound to msl2 mRNA and Sex-lethal
Published: 2014-11-07

Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR25078

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CSD1-UNR bound to msl2 mRNA and Sex-lethal

Deposition date:

2014-07-04

Original release date:

2014-11-07

Authors:

Hennig, Janosch; Wang, Iren; Sattler, Michael

Citation:

Citation: Hennig, Janosch; Militti, Cristina; Popowicz, Grzegorz; Wang, Iren; Sonntag, Miriam; Geerlof, Arie; Gabel, Frank; Gebauer, Fatima; Sattler, Michael. "Structural basis for the assembly of the Sxl-Unr translation regulatory complex" Nature 515, 287-290 (2014).
PubMed: 25209665

Assembly members:

Assembly members:
UNR, polymer, 72 residues, Formula weight is not available

Natural source:

Natural source: Common Name: fruit fly Taxonomy ID: 7227 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Drosophila melanogaster

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pTRX-CSD

Entity Sequences (FASTA):

Entity Sequences (FASTA):
UNR: GAMATRETGIIEKLLHSYGF IQCCERQARLFFHFSQFSGN IDHLKIGDPVEFEMTYDRRT GKPIASQVSKIA

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	64
13C chemical shifts	91
15N chemical shifts	62

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CSD1	1

Entities:

Entity 1, CSD1 72 residues - Formula weight is not available

1

GLY

ALA

MET

ALA

THR

ARG

GLU

THR

GLY

ILE

2

ILE

GLU

LYS

LEU

HIS

SER

TYR

GLY

PHE

3

ILE

GLN

CYS

GLU

ARG

GLN

ALA

ARG

LEU

4

PHE

HIS

PHE

SER

GLN

PHE

SER

GLY

ASN

5

ILE

ASP

HIS

LEU

LYS

ILE

GLY

ASP

PRO

VAL

6

GLU

PHE

GLU

MET

THR

TYR

ASP

ARG

THR

7

GLY

LYS

PRO

ILE

ALA

SER

GLN

VAL

SER

LYS

8

ILE

ALA

Samples:

sample_1: UNR, [U-13C; U-15N; U-2H], 0.2 – 0.5 mM; SXL-RRM1-RRM20.2 – 0.5 mM; 18-mer RNA0.2 – 0.5 mM; DTT 10 mM; potassium phosphate 10 mM; sodium chloride 50 mM; sodium azide 0.02%; sodium chloride 90%; sodium azide 10%

sample_conditions_1: temperature: 310 K; pH: 6.0; pressure: 1 atm

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D TROSY-HNCA	sample_1	isotropic	sample_conditions_1

Software:

CARA, Fred Damberger - chemical shift assignment

NMR spectrometers:

Bruker Avance 750 MHz

BMRB	25059 25060
PDB	4QQB