BMRB Entry 25021

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25021

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Title:
Backbone chemical shifts of murine Roquin-1 ROQ domain (147-326), apo form
Deposition date:
2014-06-13
Original release date:
2014-07-01
Authors:
Schlundt, Andreas; Sattler, Michael
Citation:

Citation: Schlundt, Andreas; Heinz, Gitta; Janowski, Robert; Geerlof, Arie; Stehle, Ralf; Heissmeyer, Vigo; Niessing, Dierk; Sattler, Michael. "Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation."  Nat. Struct. Mol. Biol. 21, 671-678 (2014).
PubMed: 25026077

Assembly members:

Assembly members:
ROQ_domain_of_Roquin-1, polymer, 183 residues, 20758.8 Da.

Natural source:

Natural source:   Common Name: Mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pETTrx1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ROQ_domain_of_Roquin-1: GAMEEEGRIRAMRAARSLGE RTVTELILQHQNPQQLSSNL WAAVRARGCQFLGPAMQEEA LKLVLLALEDGSALSRKVLV LFVVQRLEPRFPQASKTSIG HVVQLLYRASCFKVTKRDED SSLMQLKEEFRTYEALRREH DSQIVQIAMEAGLRIAPDQW SSLLYGDQSHKSHMQSIIDK LQT

Data sets:
Data typeCount
1H chemical shifts148
13C chemical shifts439
15N chemical shifts148

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1ROQ1

Entities:

Entity 1, ROQ 183 residues - 20758.8 Da.

starts with 147

1   GLYALAMETGLUGLUGLUGLYARGILEARG
2   ALAMETARGALAALAARGSERLEUGLYGLU
3   ARGTHRVALTHRGLULEUILELEUGLNHIS
4   GLNASNPROGLNGLNLEUSERSERASNLEU
5   TRPALAALAVALARGALAARGGLYCYSGLN
6   PHELEUGLYPROALAMETGLNGLUGLUALA
7   LEULYSLEUVALLEULEUALALEUGLUASP
8   GLYSERALALEUSERARGLYSVALLEUVAL
9   LEUPHEVALVALGLNARGLEUGLUPROARG
10   PHEPROGLNALASERLYSTHRSERILEGLY
11   HISVALVALGLNLEULEUTYRARGALASER
12   CYSPHELYSVALTHRLYSARGASPGLUASP
13   SERSERLEUMETGLNLEULYSGLUGLUPHE
14   ARGTHRTYRGLUALALEUARGARGGLUHIS
15   ASPSERGLNILEVALGLNILEALAMETGLU
16   ALAGLYLEUARGILEALAPROASPGLNTRP
17   SERSERLEULEUTYRGLYASPGLNSERHIS
18   LYSSERHISMETGLNSERILEILEASPLYS
19   LEUGLNTHR

Related Database Links:

UNP Q4VGL6
BMRB 19996
PDB 3X1O 4QI0 4QI2 4QIL 4TXA 4ULW 4YWQ
DBJ BAC23121 BAD32613 BAE24092 BAG53813
EMBL CAG07670 CDQ65000 CDQ67780 CDQ69323
GB AAH68669 AAI36785 AAI38664 AAI44409 AAI52932
REF NP_001020123 NP_001076328 NP_001084548 NP_001108155 NP_001287779
SP Q4VGL6 Q5TC82 Q6NUC6
TPG DAA21174
AlphaFold Q69Z31 Q4VGL6 Q5TC82 Q6NUC6

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks