BMRB Entry 25021

Name: Backbone chemical shifts of murine Roquin-1 ROQ domain (147-326), apo form
Published: 2014-07-01

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR25021

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone chemical shifts of murine Roquin-1 ROQ domain (147-326), apo form

Deposition date:

2014-06-13

Original release date:

2014-07-01

Authors:

Schlundt, Andreas; Sattler, Michael

Citation:

Citation: Schlundt, Andreas; Heinz, Gitta; Janowski, Robert; Geerlof, Arie; Stehle, Ralf; Heissmeyer, Vigo; Niessing, Dierk; Sattler, Michael. "Structural basis for RNA recognition in roquin-mediated post-transcriptional gene regulation." Nat. Struct. Mol. Biol. 21, 671-678 (2014).
PubMed: 25026077

Assembly members:

Assembly members:
ROQ_domain_of_Roquin-1, polymer, 183 residues, 20758.8 Da.

Natural source:

Natural source: Common Name: Mouse Taxonomy ID: 10090 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Mus musculus

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pETTrx1a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
ROQ_domain_of_Roquin-1: GAMEEEGRIRAMRAARSLGE RTVTELILQHQNPQQLSSNL WAAVRARGCQFLGPAMQEEA LKLVLLALEDGSALSRKVLV LFVVQRLEPRFPQASKTSIG HVVQLLYRASCFKVTKRDED SSLMQLKEEFRTYEALRREH DSQIVQIAMEAGLRIAPDQW SSLLYGDQSHKSHMQSIIDK LQT

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
1H chemical shifts	148
13C chemical shifts	439
15N chemical shifts	148

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	ROQ	1

Entities:

Entity 1, ROQ 183 residues - 20758.8 Da.

starts with 147

1

GLY

ALA

MET

GLU

GLY

ARG

ILE

ARG

2

ALA

MET

ARG

ALA

ARG

SER

LEU

GLY

GLU

3

ARG

THR

VAL

THR

GLU

LEU

ILE

LEU

GLN

HIS

4

GLN

ASN

PRO

GLN

LEU

SER

ASN

LEU

5

TRP

ALA

VAL

ARG

ALA

ARG

GLY

CYS

GLN

6

PHE

LEU

GLY

PRO

ALA

MET

GLN

GLU

ALA

7

LEU

LYS

LEU

VAL

LEU

ALA

LEU

GLU

ASP

8

GLY

SER

ALA

LEU

SER

ARG

LYS

VAL

LEU

VAL

9

LEU

PHE

VAL

GLN

ARG

LEU

GLU

PRO

ARG

10

PHE

PRO

GLN

ALA

SER

LYS

THR

SER

ILE

GLY

11

HIS

VAL

GLN

LEU

TYR

ARG

ALA

SER

12

CYS

PHE

LYS

VAL

THR

LYS

ARG

ASP

GLU

ASP

13

SER

LEU

MET

GLN

LEU

LYS

GLU

PHE

14

ARG

THR

TYR

GLU

ALA

LEU

ARG

GLU

HIS

15

ASP

SER

GLN

ILE

VAL

GLN

ILE

ALA

MET

GLU

16

ALA

GLY

LEU

ARG

ILE

ALA

PRO

ASP

GLN

TRP

17

SER

LEU

TYR

GLY

ASP

GLN

SER

HIS

18

LYS

SER

HIS

MET

GLN

SER

ILE

ASP

LYS

19

LEU

GLN

THR

Samples:

sample_1: ROQ domain of Roquin-1, [U-98% 13C; U-98% 15N], 200 – 350 uM; TRIS, pH 7.0 20 mM; TCEP 2 mM; sodium chloride 150 mM

sample_conditions_1: temperature: 298 K; pH: 7.0; pressure: 1 atm; ionic strength: 150 mM

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CCPN_Analysis v2, CCPN - chemical shift assignment

NMR spectrometers:

Bruker Avance 600 MHz
Bruker Avance 800 MHz

UNP	Q4VGL6
BMRB	19996
PDB	3X1O 4QI0 4QI2 4QIL 4TXA 4ULW 4YWQ
DBJ	BAC23121 BAD32613 BAE24092 BAG53813
EMBL	CAG07670 CDQ65000 CDQ67780 CDQ69323
GB	AAH68669 AAI36785 AAI38664 AAI44409 AAI52932
REF	NP_001020123 NP_001076328 NP_001084548 NP_001108155 NP_001287779
SP	Q4VGL6 Q5TC82 Q6NUC6
TPG	DAA21174
AlphaFold	Q69Z31 Q4VGL6 Q5TC82 Q6NUC6