BMRB Entry 19819

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19819

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Title:
Murine Neuroglobin, Fe3+ form, liganded to cyanide (cyanometNgB)
Deposition date:
2014-02-26
Original release date:
2015-03-05
Authors:
Yang, Yin Shan; Allemand, Frederic; Guca, Ewelina; Vallone, Beatrice; Delbecq, Stephane; Roumestand, Christian
Citation:

Citation: Yang, Yin Shan; Allemand, Frederic; Guca, Ewelina; Vallone, Beatrice; Delbecq, Stephane; Roumestand, Christian. "1H, 15N and 13C Backbone resonance assignments of murine met-Neuroglobin, free and in complex with cyanide."  Biomol. NMR Assignments ., .-..

Assembly members:

Assembly members:
cyanometNgb, polymer, 151 residues, Formula weight is not available
CYANIDE ION, non-polymer, 26.017 Da.

Natural source:

Natural source:   Common Name: House mouse   Taxonomy ID: 10090   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Mus musculus

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET15b-tev

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cyanometNgb: MERPESELIRQSWRVVSRSP LEHGTVLFARLFALEPSLLP LFQYNGRQFSSPEDSLSSPE FLDHIRKVMLVIDAAVTNVE DLSSLEEYLTSLGRKHRAVG VRLSSFSTVGESLLYMLEKS LGPDFTPATRTAWSRLYGAV VQAMSRGWDGE

Data sets:
Data typeCount
13C chemical shifts849
15N chemical shifts272
1H chemical shifts579

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1cyanometNgb1
2cyanide ion2

Entities:

Entity 1, cyanometNgb 151 residues - Formula weight is not available

1   METGLUARGPROGLUSERGLULEUILEARG
2   GLNSERTRPARGVALVALSERARGSERPRO
3   LEUGLUHISGLYTHRVALLEUPHEALAARG
4   LEUPHEALALEUGLUPROSERLEULEUPRO
5   LEUPHEGLNTYRASNGLYARGGLNPHESER
6   SERPROGLUASPSERLEUSERSERPROGLU
7   PHELEUASPHISILEARGLYSVALMETLEU
8   VALILEASPALAALAVALTHRASNVALGLU
9   ASPLEUSERSERLEUGLUGLUTYRLEUTHR
10   SERLEUGLYARGLYSHISARGALAVALGLY
11   VALARGLEUSERSERPHESERTHRVALGLY
12   GLUSERLEULEUTYRMETLEUGLULYSSER
13   LEUGLYPROASPPHETHRPROALATHRARG
14   THRALATRPSERARGLEUTYRGLYALAVAL
15   VALGLNALAMETSERARGGLYTRPASPGLY
16   GLU

Entity 2, cyanide ion - C N - 26.017 Da.

1   CYN

Samples:

sample_1: cyanometNgb, [U-100% 13C; U-100% 15N], 0.3 mM; Tris, HCl 50 mM; D2O 5%; H2O 90%; PMSF 0.05 mM; DSS 0.05 mM

sample_conditions_1: pH: 7.0; pressure: ambient atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-15N TOCSYsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HN(CA)COsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-13C HSQCsample_1isotropicsample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection

NMR spectrometers:

  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 19818
PDB
DBJ BAE38375
EMBL CAC11135 CAE30466
GB AAH24263 EDL02920
REF NP_071859
SP Q9ER97
AlphaFold Q9ER97

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks