BMRB Entry 19816

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PDB ID: 2mle
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19816
MolProbity Validation Chart

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Title:
NMR structure of the C-domain of troponin C bound to the anchoring region of troponin I
Deposition date:
2014-02-26
Original release date:
2014-03-10
Authors:
Robertson, Ian; Baryshnikova, Olga; Mercier, Pascal; Sykes, Brian
Citation:

Citation: Baryshnikova, Olga; Robertson, Ian; Mercier, Pascal; Sykes, Brian. "The dilated cardiomyopathy G159D mutation in cardiac troponin C weakens the anchoring interaction with troponin I."  Biochemistry 47, 10950-10960 (2008).
PubMed: 18803402

Assembly members:

Assembly members:
cCTnC, polymer, 72 residues, 8369.260 Da.
CALCIUM ION, non-polymer, 40.078 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: BL21(DE3)pLysS

Entity Sequences (FASTA):

Entity Sequences (FASTA):
cCTnC: MGKSEEELSDLFRMFDKNAD GYIDLDELKIMLQATGETIT EDDIEELMKDGDKNNDGRID YDEFLEFMKGVE

Data sets:
Data typeCount
1H chemical shifts403
13C chemical shifts216
15N chemical shifts70

Additional metadata:

  • Assembly
  • Samples and Experiments
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Assembly:

Entity Assembly IDEntity NameEntity ID
1cCTnC1
2Calcium_12
3Calcium_22

Entities:

Entity 1, cCTnC 72 residues - 8369.260 Da.

1   METGLYLYSSERGLUGLUGLULEUSERASP
2   LEUPHEARGMETPHEASPLYSASNALAASP
3   GLYTYRILEASPLEUASPGLULEULYSILE
4   METLEUGLNALATHRGLYGLUTHRILETHR
5   GLUASPASPILEGLUGLULEUMETLYSASP
6   GLYASPLYSASNASNASPGLYARGILEASP
7   TYRASPGLUPHELEUGLUPHEMETLYSGLY
8   VALGLU

Entity 2, Calcium_1 - Ca - 40.078 Da.

1   CA

Related Database Links:

BMRB 15385 15388 15400 15427 16752 17243 17440 19817 25120
PDB 1AJ4 1DTL 1FI5 1IH0 1J1D 1J1E 1LA0 1OZS 1SBJ 1SCV 2JT0 2JT3 2JT8 2JTZ 2KDH 2L98 2MLE 2MLF 3CTN
DBJ BAA02369 BAG36483
EMBL CAA30736 CAG46663 CAG46683
GB AAA36772 AAA37492 AAA37493 AAA48654 AAB91994
PIR TPHUCC
PRF 1510257A 750650A
REF NP_001029277 NP_001029523 NP_001123715 NP_001272501 NP_001291793
SP P02591 P09860 P19123 P63315 P63316
TPG DAA16908
AlphaFold P02591 P09860 P19123 P63315 P63316

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