BMRB Entry 19729
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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19729
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Herve, Virginie; Meudal, Herve; Labas, Valerie; Rehault Godbert, Sophie; Gautron, Joel; Berges, Magali; Guyot, Nicolas; Delmas, Agnes; Nys, Yves; Landon, Celine. "3D NMR structure of hen egg gallin (chicken ovo-defensin) reveals a new variation of the beta-defensin fold." J. Biol. Chem. ., .-. (2014).
PubMed: 24443564
Assembly members:
GALLIN_PROTEIN, polymer, 41 residues, 4738.724 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
GALLIN_PROTEIN: LVLKYCPKIGYCSNTCSKTQ
IWATSHGCKMYCCLPASWKW
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 289 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | GALLIN PROTEIN | 1 |
Entities:
Entity 1, GALLIN PROTEIN 41 residues - 4738.724 Da.
| 1 | LEU | VAL | LEU | LYS | TYR | CYS | PRO | LYS | ILE | GLY | ||||
| 2 | TYR | CYS | SER | ASN | THR | CYS | SER | LYS | THR | GLN | ||||
| 3 | ILE | TRP | ALA | THR | SER | HIS | GLY | CYS | LYS | MET | ||||
| 4 | TYR | CYS | CYS | LEU | PRO | ALA | SER | TRP | LYS | TRP | ||||
| 5 | LYS |
Samples:
sample_1: GALLIN PROTEIN 1 mM
sample_2: GALLIN PROTEIN 1 mM
sample_conditions_1: ionic strength: 0 mM; pH: 5.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-13C HSQC | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA1.1, LINGE, O'DONOGHUE - refinement
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - structure solution
NMRVIEW, Johnson, One Moon Scientific - structure solution
NMR spectrometers:
- VARIAN INOVA 600 MHz