BMRB Entry 17823
Chem Shift validation: AVS_anomalous, AVS_full, SPARTA
BMRB Entry DOI: doi:10.13018/BMR17823
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Citation: Herve, Virginie; Meudal, Herve; Labas, Valerie; Rehault-Godbert, Sophie; Gautron, Joel; Berges, Magali; Guyot, Nicolas; Delmas, Agnes; Nys, Yves; Landon, Celine. "Three-dimensional NMR Structure of Hen Egg Gallin (Chicken Ovodefensin) Reveals a New Variation of the -Defensin Fold." J. Biol. Chem. 289, 7211-7220 (2014).
PubMed: 24443564
Assembly members:
entity, polymer, 41 residues, 4738.724 Da.
Natural source: Common Name: chicken Taxonomy ID: 9031 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Gallus gallus
Experimental source: Production method: chemical synthesis
Entity Sequences (FASTA):
entity: LVLKYCPKIGYCSNTCSKTQ
IWATSHGCKMYCCLPASWKW
K
- assigned_chemical_shifts
| Data type | Count |
| 1H chemical shifts | 289 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | AvBD15 (GALLIN) | 1 |
Entities:
Entity 1, AvBD15 (GALLIN) 41 residues - 4738.724 Da.
| 1 | LEU | VAL | LEU | LYS | TYR | CYS | PRO | LYS | ILE | GLY | ||||
| 2 | TYR | CYS | SER | ASN | THR | CYS | SER | LYS | THR | GLN | ||||
| 3 | ILE | TRP | ALA | THR | SER | HIS | GLY | CYS | LYS | MET | ||||
| 4 | TYR | CYS | CYS | LEU | PRO | ALA | SER | TRP | LYS | TRP | ||||
| 5 | LYS |
Samples:
sample_1: entity 1.1 mM
sample_2: entity 1.1 mM
sample_conditions_1: ionic strength: 0 M; pH: 5.5; pressure: 1 atm; temperature: 293 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D DQF-COSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_1 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_1 | isotropic | sample_conditions_1 |
| 2D DQF-COSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H TOCSY | sample_2 | isotropic | sample_conditions_1 |
| 2D 1H-1H NOESY | sample_2 | isotropic | sample_conditions_1 |
Software:
NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing
ARIA, Linge, O'Donoghue and Nilges - refinement, structure solution
NMRView, Johnson, One Moon Scientific - chemical shift assignment, peak picking
NMR spectrometers:
- Varian INOVA 600 MHz