BMRB Entry 19713

Name: Solution Structure of Domain-Swapped GLPG
Published: 2014-11-10

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PDB ID: 2mja
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19713
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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Solution Structure of Domain-Swapped GLPG

Deposition date:

2013-12-31

Original release date:

2014-11-10

Authors:

Ghasriani, Houman; Kwok, Jason; Sherrat, Allison; Goto, Natalie

Citation:

Citation: Ghasriani, Houman; Kwok, Jason; Sherrat, Allison; Foo, Alexander; Qureshi, Tabussom; Goto, Natalie. "Micelle-Catalyzed Domain Swapping in the GlpG Rhomboid Protease Cytoplasmic Domain" Biochemistry 53, 5907-5915 (2014).
PubMed: 25162988

Assembly members:

Assembly members:
Domain_Swapped_GLPG, polymer, 63 residues, 7156.092 Da.

Natural source:

Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: PET 30A +

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Domain_Swapped_GLPG: MLMITSFANPRVAQAFVDYM ATQGVILTIQQHNQSDVWLA DESQAERVRAELARFLENPA DLE

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	193
15N chemical shifts	69
1H chemical shifts	429

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Domain_Swapped_GLPG_1	1
2	Domain_Swapped_GLPG_2	1

Entities:

Entity 1, Domain_Swapped_GLPG_1 63 residues - 7156.092 Da.

1

MET

LEU

MET

ILE

THR

SER

PHE

ALA

ASN

PRO

2

ARG

VAL

ALA

GLN

ALA

PHE

VAL

ASP

TYR

MET

3

ALA

THR

GLN

GLY

VAL

ILE

LEU

THR

ILE

GLN

4

GLN

HIS

ASN

GLN

SER

ASP

VAL

TRP

LEU

ALA

5

ASP

GLU

SER

GLN

ALA

GLU

ARG

VAL

ARG

ALA

6

GLU

LEU

ALA

ARG

PHE

LEU

GLU

ASN

PRO

ALA

7

ASP

LEU

GLU

Samples:

sample_1: Domain Swapped GLPG, [U-100% 13C; U-100% 15N], 1 mM; NaH2PO4 25 mM; NaCl 150 mM; H2O 90%; D2O, [U-100% 2H], 10%

sample_conditions_1: ionic strength: 0.3 M; pH: 6.5; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HCCH-TOCSY	sample_1	isotropic	sample_conditions_1
3D 1H-13C NOESY aliphatic	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

CYANA v2.1, Guntert, Mumenthaler and Wuthrich - structure solution

NMR spectrometers:

Varian INOVA 500 MHz
Varian INOVA 800 MHz

NCBI	ECs4267
BMRB	17720
PDB	2LEP 2MJA
DBJ	BAB37690 BAE77868 BAG79214 BAI27681 BAI32850
EMBL	CAP77869 CAQ33744 CAR00366 CAR05025 CAR10074
GB	AAG58528 AAN44906 AAN82639 AAP19275 AAT48182
REF	NP_312294 NP_709199 WP_000541600 WP_000928708 WP_000928709
SP	A1AGU7 A7ZSV4 A8A5N2 A8AQX4 B1IP42
AlphaFold	A1AGU7 A7ZSV4 A8A5N2 A8AQX4 B1IP42