BMRB Entry 19645

Name: CD79a cytosolic domain denatured
Published: 2014-02-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19645

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CD79a cytosolic domain denatured

Deposition date:

2013-12-02

Original release date:

2014-02-11

Authors:

Rosenlow, Joakim

Citation:

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Rosenlow, Joakim; Brutscher, Bernhard; Karlsson, B. Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains." PLoS ONE 8, e62947-e62947 (2013).
PubMed: 23667548

Assembly members:

Assembly members:
CD79a_cyt, polymer, 63 residues, 7188.7093 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: E. coli - cell free Vector: pEXP5-NT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD79a_cyt: SLRKRWQNEKLGLDAGDEYE DENLYEGLNLDDCSMYEDIS RGLQGTYQDVGSLNIGDVQL EKP

Data sets:

assigned_chemical_shifts
- CD79a_cyt__denatured_assigned_chem_shift_list

Data type	Count
13C chemical shifts	173
15N chemical shifts	60
1H chemical shifts	60

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD79a_cyt	1

Entities:

Entity 1, CD79a_cyt 63 residues - 7188.7093 Da.

1

SER

LEU

ARG

LYS

ARG

TRP

GLN

ASN

GLU

LYS

2

LEU

GLY

LEU

ASP

ALA

GLY

ASP

GLU

TYR

GLU

3

ASP

GLU

ASN

LEU

TYR

GLU

GLY

LEU

ASN

LEU

4

ASP

CYS

SER

MET

TYR

GLU

ASP

ILE

SER

5

ARG

GLY

LEU

GLN

GLY

THR

TYR

GLN

ASP

VAL

6

GLY

SER

LEU

ASN

ILE

GLY

ASP

VAL

GLN

LEU

7

GLU

LYS

PRO

Samples:

Denatured: CD79a_cyt, [U-99% 13C; U-99% 15N], 200 – 300 uM; DTT 2 mM; sodium phosphate 20 mM; azide 0.02%; Complete EDTA-free 1 x; sodium chloride 150 mM; TSP 100 uM; urea 6 M; H2O 90%; D2O, [U-100% 2H], 10%

Denatured_1: pH: 7.000; pressure: 1.000 atm; temperature: 288.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Denatured	isotropic	Denatured_1
3D HN(ca)CO	Denatured	isotropic	Denatured_1
3D HNCA	Denatured	isotropic	Denatured_1
3D HNCACB	Denatured	isotropic	Denatured_1
3D HNCO	Denatured	isotropic	Denatured_1
3D HN(CO)CA	Denatured	isotropic	Denatured_1
3D HN(COCA)CB	Denatured	isotropic	Denatured_1

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - Spectrum analysis

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing

TANSY v1.0, (MDDNMR) Mayzel, Orekhov - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

Varian UnityInova 600 MHz

UniProt	P11912
BMRB	18867 19644 19648 19649 19723 19724
DBJ	BAG37737
EMBL	CAA31802 CAA58523
GB	AAA20495 AAA57274 AAA59556 AAA59557 AAA60270
REF	NP_001774 NP_067612 XP_001152742 XP_002829327 XP_003779238
SP	P11912
AlphaFold	Q53FB8 P11912