BMRB Entry 18867

Name: CD79a cytosolic domain
Published: 2013-10-15

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR18867

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

CD79a cytosolic domain

Deposition date:

2012-11-29

Original release date:

2013-10-15

Authors:

Mayzel, Maxim

Citation:

Citation: Isaksson, Linnea; Mayzel, Maxim; Saline, Maria; Pedersen, Anders; Brutscher, Bernhard; Karlsson, Goran; Orekhov, Vladislav. "Highly efficient NMR assignment of intrinsically disordered proteins: application to B- and T cell receptor domains" PLoS One 8, e62947-e62947 (2013).
PubMed: 23667548

Assembly members:

Assembly members:
CD79a, polymer, 63 residues, 7188.7093 Da.

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: cell free synthesis Host organism: Wheat germ - cell freeE. coli - cell free Vector: pEXP5-NT

Entity Sequences (FASTA):

Entity Sequences (FASTA):
CD79a: SLRKRWQNEKLGLDAGDEYE DENLYEGLNLDDCSMYEDIS RGLQGTYQDVGSLNIGDVQL EKP

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1
- assigned_chem_shift_list_2

Data type	Count
13C chemical shifts	351
15N chemical shifts	120
1H chemical shifts	120

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	CD79a, native	1
2	CD79a, denatured	1

Entities:

Entity 1, CD79a, native 63 residues - 7188.7093 Da.

1

SER

LEU

ARG

LYS

ARG

TRP

GLN

ASN

GLU

LYS

2

LEU

GLY

LEU

ASP

ALA

GLY

ASP

GLU

TYR

GLU

3

ASP

GLU

ASN

LEU

TYR

GLU

GLY

LEU

ASN

LEU

4

ASP

CYS

SER

MET

TYR

GLU

ASP

ILE

SER

5

ARG

GLY

LEU

GLN

GLY

THR

TYR

GLN

ASP

VAL

6

GLY

SER

LEU

ASN

ILE

GLY

ASP

VAL

GLN

LEU

7

GLU

LYS

PRO

Samples:

Nativ: CD79a, [U-99% 13C; U-99% 15N], 0.1 ± 1e-05 mM; DTT 2 mM; EDTA 1 mM; potassium phosphate 20 mM; azide 0.02%; Complete EDTA-free 1 x; H2O 90%; D2O 10%

Denatured: CD79a, [U-99% 13C; U-99% 15N], 0.1 mM; DTT 2 mM; EDTA 1 mM; potassium phosphate 20 mM; azide 0.02%; Complete EDTA-free 1 x; UREA 6 M; H2O 90%; D2O 10%

Normal: ionic strength: 0.020 M; pH: 6.700; pressure: 1.000 atm; temperature: 298.000 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	Nativ	isotropic	Normal
3D HN(ca)CO	Nativ	isotropic	Normal
3D HNCA	Nativ	isotropic	Normal
3D HNCACB	Nativ	isotropic	Normal
3D HNCO	Nativ	isotropic	Normal
3D HN(CO)CA	Nativ	isotropic	Normal
3D HN(COCA)CB	Nativ	isotropic	Normal
2D 1H-15N HSQC	Denatured	isotropic	Normal
3D HN(ca)CO	Denatured	isotropic	Normal
3D HNCA	Denatured	isotropic	Normal
3D HNCACB	Denatured	isotropic	Normal
3D HNCO	Denatured	isotropic	Normal
3D HN(CO)CA	Denatured	isotropic	Normal
3D HN(COCA)CB	Denatured	isotropic	Normal

Software:

AutoAssign v2.3.0, Zimmerman, Moseley, Kulikowski and Montelione - Automatic assignment

ANALYSIS v2.2, CCPN - Spectrum analysis

MDDNMR v2.1, (MDDNMR) Orekhov, Jaravine, Mayzel, Kazimierczuk - Spectrum processing

TANSY v1.0, (MDDNMR) Mayzel, Orekhov - Spectra processing & analysis

NMRPipe vany, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - Spectrum processing

NMR spectrometers:

Varian UnityInova 600 MHz

BMRB	19644 19645 19648 19649 19723 19724
DBJ	BAG37737
EMBL	CAA31802 CAA58523
GB	AAA20495 AAA57274 AAA59556 AAA59557 AAA60270
REF	NP_001774 NP_067612 XP_001152742 XP_002829327 XP_003779238
SP	P11912
AlphaFold	P11912