BMRB Entry 19576

Name: Backbone resonance assignment of FAPP1 PH domain in the presence of 10% (w/v) DMPC/DHPC (q=0.25), and in the presence of 10% (w/v) DMPC/DHPC (q=0.25) plus 8mM PI4P
Published: 2014-02-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19576

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

Backbone resonance assignment of FAPP1 PH domain in the presence of 10% (w/v) DMPC/DHPC (q=0.25), and in the presence of 10% (w/v) DMPC/DHPC (q=0.25) plus 8mM PI4P

Deposition date:

2013-10-23

Original release date:

2014-02-11

Authors:

Prestegard, James; Liu, Yizhou

Citation:

Citation: Liu, Yizhou; Kahn, Richard; Prestegard, James. "Interaction of Fapp1 with Arf1 and PI4P at a membrane surface: an example of coincidence detection." Structure 22, 421-430 (2014).
PubMed: 24462251

Assembly members:

Assembly members:
FAPP1, polymer, 106 residues, Formula weight is not available

Natural source:

Natural source: Common Name: Human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET20(b)

Entity Sequences (FASTA):

Entity Sequences (FASTA):
FAPP1: MEGVLYKWTNYLTGWQPRWF VLDNGILSYYDSQDDVCKGS KGSIKMAVCEIKVHSADNTR MELIIPGEQHFYMKAVNAAE RQRWLVALGSSKACLTDTLE HHHHHH

Data sets:

assigned_chemical_shifts
- FAPP1_with_DMPC-DHPC
- FAPP1_with_DMPC-DHPC_and_PI4P

Data type	Count
13C chemical shifts	449
15N chemical shifts	191
1H chemical shifts	191

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
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Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	FAPP1 PH domain	1

Entities:

Entity 1, FAPP1 PH domain 106 residues - Formula weight is not available

1

MET

GLU

GLY

VAL

LEU

TYR

LYS

TRP

THR

ASN

2

TYR

LEU

THR

GLY

TRP

GLN

PRO

ARG

TRP

PHE

3

VAL

LEU

ASP

ASN

GLY

ILE

LEU

SER

TYR

4

ASP

SER

GLN

ASP

VAL

CYS

LYS

GLY

SER

5

LYS

GLY

SER

ILE

LYS

MET

ALA

VAL

CYS

GLU

6

ILE

LYS

VAL

HIS

SER

ALA

ASP

ASN

THR

ARG

7

MET

GLU

LEU

ILE

PRO

GLY

GLU

GLN

HIS

8

PHE

TYR

MET

LYS

ALA

VAL

ASN

ALA

GLU

9

ARG

GLN

ARG

TRP

LEU

VAL

ALA

LEU

GLY

SER

10

SER

LYS

ALA

CYS

LEU

THR

ASP

THR

LEU

GLU

11

HIS

Samples:

sample_1: FAPP1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; DMPC/DHPC 10 % (w/v); K2HPO4-KH2PO4 10 mM; NaCl 50 mM; K2SO4 10 mM; MgCl2 2 mM; DTT 5 mM

sample_2: FAPP1, [U-100% 13C; U-100% 15N; U-80% 2H], 0.8 mM; DMPC/DHPC 10 % (w/v); Pi4P 8 mM; K2HPO4-KH2PO4 10 mM; NaCl 50 mM; K2SO4 10 mM; MgCl2 2 mM; DTT 5 mM

sample_conditions_1: ionic strength: 0.1 M; pH: 7.0; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
2D 1H-15N HSQC	sample_2	isotropic	sample_conditions_1
3D HNCACB	sample_2	isotropic	sample_conditions_1

Software:

VNMRJ, Varian - collection

NMRPipe, Delaglio, Grzesiek, Vuister, Zhu, Pfeifer and Bax - processing

SPARKY, Goddard - chemical shift assignment

NMR spectrometers:

Varian VNMRS 900 MHz

BMRB	16082 19508
PDB	2KCJ 2MDX
DBJ	BAG37067
GB	AAG15199 AAG15200 AAH31110 AAH44567 AAH83759
REF	NP_001013095 NP_001095383 NP_001230393 NP_061964 NP_112546
SP	Q9ERS4 Q9HB20
TPG	DAA32821
AlphaFold	Q9ERS4 Q9HB20