BMRB Entry 19508

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS, SPARTA
BMRB Entry DOI: doi:10.13018/BMR19508
MolProbity Validation Chart

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Title:
Solution structure of the human wild type FAPP1-PH domain
Deposition date:
2013-09-19
Original release date:
2014-09-22
Authors:
Lenoir, Marc; Overduin, Michael
Citation:

Citation: Lenoir, Marc; Overduin, Michael. "Solution structure of the human wild type FAPP1-PH domain"  .

Assembly members:

Assembly members:
entity, polymer, 108 residues, 12236.042 Da.

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pGex

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity: GPLGSPEFMEGVLYKWTNYL TGWQPRWFVLDNGILSYYDS QDDVCKGSKGSIKMAVCEIK VHSADNTRMELIIPGEQHFY MKAVNAAERQRWLVALGSSK ACLTDTRT

Data sets:
Data typeCount
13C chemical shifts400
15N chemical shifts113
1H chemical shifts688

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
  • Hide all

Assembly:

Entity Assembly IDEntity NameEntity ID
1human wild type FAPP1-PH domain1

Entities:

Entity 1, human wild type FAPP1-PH domain 108 residues - 12236.042 Da.

1   GLYPROLEUGLYSERPROGLUPHEMETGLU
2   GLYVALLEUTYRLYSTRPTHRASNTYRLEU
3   THRGLYTRPGLNPROARGTRPPHEVALLEU
4   ASPASNGLYILELEUSERTYRTYRASPSER
5   GLNASPASPVALCYSLYSGLYSERLYSGLY
6   SERILELYSMETALAVALCYSGLUILELYS
7   VALHISSERALAASPASNTHRARGMETGLU
8   LEUILEILEPROGLYGLUGLNHISPHETYR
9   METLYSALAVALASNALAALAGLUARGGLN
10   ARGTRPLEUVALALALEUGLYSERSERLYS
11   ALACYSLEUTHRASPTHRARGTHR

Samples:

sample_1: entity, [U-13C; U-15N], 500 – 800 uM; DTT 1 mM; TRIS 20 mM; sodium chloride 100 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 240 mM; pH: 7; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1

Software:

ANALYSIS v2.2, CCPN - assignment

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Varian INOVA 800 MHz
  • Varian INOVA 900 MHz

Related Database Links:

BMRB 16082 19576
PDB
DBJ BAG37067
GB AAG15199 AAG15200 AAH31110 AAH44567 AAH83759
REF NP_001013095 NP_001095383 NP_001230393 NP_061964 NP_112546
SP Q9ERS4 Q9HB20
TPG DAA32821
AlphaFold Q9ERS4 Q9HB20

Download HSQC peak lists in one of the following formats:
CSV: Backbone or all simulated peaks
SPARKY: Backbone or all simulated peaks