BMRB Entry 19429

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full
BMRB Entry DOI: doi:10.13018/BMR19429
MolProbity Validation Chart

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Title:
A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement
Deposition date:
2013-08-15
Original release date:
2014-02-12
Authors:
Liu, Bing; Shadrin, Andrey; Sheppard, Carol; Xu, Yingqi; Severinov, Konstantin; Matthews, Steve; Wigneshweraraj, Sivaramesh
Citation:

Citation: Liu, Bing; Shadrin, Andrey; Sheppard, Carol; Mekler, Vladimir; Xu, Yingqi; Severinov, Konstantin; Matthews, Steve; Wigneshweraraj, Sivaramesh. "A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement."  Nucleic Acids Res. 42, 4294-4305 (2014).
PubMed: 24482445

Assembly members:

Assembly members:
entity_1, polymer, 67 residues, 8189.045 Da.
entity_2, polymer, 8 residues, 1236.468 Da.

Natural source:

Natural source:   Common Name: E. coli   Taxonomy ID: 562   Superkingdom: Bacteria   Kingdom: not available   Genus/species: Escherichia coli

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pet46

Entity Sequences (FASTA):

Entity Sequences (FASTA):
entity_1: NEFTQISGYVNAFGSQRGSV LTVKVENDEGWTLVEEDFDR ADYGSDPEFVAEVSSYLKRN GGIKDLT
entity_2: DLLNLFNQ

Data sets:
Data typeCount
13C chemical shifts289
15N chemical shifts66
1H chemical shifts464

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1entity_11
2entity_22

Entities:

Entity 1, entity_1 67 residues - 8189.045 Da.

1   ASNGLUPHETHRGLNILESERGLYTYRVAL
2   ASNALAPHEGLYSERGLNARGGLYSERVAL
3   LEUTHRVALLYSVALGLUASNASPGLUGLY
4   TRPTHRLEUVALGLUGLUASPPHEASPARG
5   ALAASPTYRGLYSERASPPROGLUPHEVAL
6   ALAGLUVALSERSERTYRLEULYSARGASN
7   GLYGLYILELYSASPLEUTHR

Entity 2, entity_2 8 residues - 1236.468 Da.

1   ASPLEULEUASNLEUPHEASNGLN

Samples:

sample_1: sodium chloride 5 mM; potassium phosphate 5 mM; entity_1 5 mM; entity_2 5 mM; H2O 90%; D2O 10%

sample_2: sodium chloride 5 mM; potassium phosphate 5 mM; entity_1 5 mM; entity_2 5 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 10 mM; pH: 8.7; pressure: 1 atm; temperature: 283 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D 1H-13C NOESYsample_1isotropicsample_conditions_1
3D HBHA(CO)NHsample_1isotropicsample_conditions_1
3D HCCH-TOCSYsample_1isotropicsample_conditions_1
2D 1H-1H COSYsample_2isotropicsample_conditions_1
2D 1H-1H NOESYsample_2isotropicsample_conditions_1

Software:

ARIA, Linge, O'Donoghue and Nilges - structure solution

NMR spectrometers:

  • Bruker DRX 800 MHz
  • Bruker DRX 600 MHz

Related Database Links:

BMRB 19428
PDB
GB AAM74072 AAP58713
REF NP_858993
AlphaFold Q5GWS7

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