BMRB Entry 19428
Click here to enlarge.
PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_anomalous, AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19428
MolProbity Validation Chart
NMR-STAR file interactive viewer.
NMR-STAR v3 text file.
XML gzip file.
RDF gzip file.
All files associated with the entry
Citation: Liu, Bing; Shadrin, Andrey; Sheppard, Carol; Mekler, Vladimir; Xu, Yingqi; Severinov, Konstantin; Matthews, Steve; Wigneshweraraj, Sivaramesh. "A bacteriophage transcription regulator inhibits bacterial transcription initiation by -factor displacement." Nucleic Acids Res. 42, 4294-4305 (2014).
PubMed: 24482445
Assembly members:
entity, polymer, 71 residues, 8189.045 Da.
Natural source: Common Name: E. coli Taxonomy ID: 562 Superkingdom: Bacteria Kingdom: not available Genus/species: Escherichia coli
Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pet46
Entity Sequences (FASTA):
entity: MNEFTQISGYVNAFGSQRGS
VLTVKVENDEGWTLVEEDFD
RADYGSDPEFVAEVSSYLKR
NGGIKDLTKVL
- assigned_chemical_shifts
| Data type | Count |
| 13C chemical shifts | 272 |
| 15N chemical shifts | 64 |
| 1H chemical shifts | 418 |
Additional metadata:
Assembly:
| Entity Assembly ID | Entity Name | Entity ID |
|---|---|---|
| 1 | entity | 1 |
Entities:
Entity 1, entity 71 residues - 8189.045 Da.
| 1 | MET | ASN | GLU | PHE | THR | GLN | ILE | SER | GLY | TYR | ||||
| 2 | VAL | ASN | ALA | PHE | GLY | SER | GLN | ARG | GLY | SER | ||||
| 3 | VAL | LEU | THR | VAL | LYS | VAL | GLU | ASN | ASP | GLU | ||||
| 4 | GLY | TRP | THR | LEU | VAL | GLU | GLU | ASP | PHE | ASP | ||||
| 5 | ARG | ALA | ASP | TYR | GLY | SER | ASP | PRO | GLU | PHE | ||||
| 6 | VAL | ALA | GLU | VAL | SER | SER | TYR | LEU | LYS | ARG | ||||
| 7 | ASN | GLY | GLY | ILE | LYS | ASP | LEU | THR | LYS | VAL | ||||
| 8 | LEU |
Samples:
sample_1: entity 5 mM; sodium chloride 5 mM; potassium phosphate 5 mM; H2O 90%; D2O 10%
sample_conditions_1: ionic strength: 10 mM; pH: 8.7; pressure: 1 atm; temperature: 283 K
Experiments:
| Name | Sample | Sample state | Sample conditions |
|---|---|---|---|
| 2D 1H-15N HSQC | sample_1 | isotropic | sample_conditions_1 |
| 3D CBCA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCO | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCA | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-15N NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HNCACB | sample_1 | isotropic | sample_conditions_1 |
| 3D 1H-13C NOESY | sample_1 | isotropic | sample_conditions_1 |
| 3D HBHA(CO)NH | sample_1 | isotropic | sample_conditions_1 |
| 3D HCCH-TOCSY | sample_1 | isotropic | sample_conditions_1 |
Software:
ARIA, Linge, O'Donoghue and Nilges - structure solution
NMR spectrometers:
- Bruker DRX 800 MHz
- Bruker DRX 600 MHz
Download HSQC peak lists in one of the following formats:
CSV: Backbone
or all simulated peaks
SPARKY: Backbone
or all simulated peaks