BMRB Entry 19320

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PDB ID:
Entry in NMR Restraints Grid
Validation report in NRG-CING
Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19320
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Title:
1H, 13C and 15N NMR assignments of RNA recognition motif of human Taf15
Deposition date:
2013-06-25
Original release date:
2015-03-23
Authors:
Kashyap, Maruthi; Ganguly, Akshay; Bhavesh, Neel Sarovar
Citation:

Citation: Kashyap, Maruthi; Ganguly, Akshay; Bhavesh, Neel Sarovar. "1H, 13C and 15N NMR assignments of RNA recognition motif of human Taf15"  .

Assembly members:

Assembly members:
Taf15, polymer, 97 residues, Formula weight is not available

Natural source:

Natural source:   Common Name: Human   Taxonomy ID: 9606   Superkingdom: Eukaryota   Kingdom: Metazoa   Genus/species: Homo sapiens

Experimental source:

Experimental source:   Production method: recombinant technology   Host organism: Escherichia coli   Vector: pET28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Taf15: GSHMSDNNTIFVQGLGEGVS TDQVGEFFKQIGIIKTNKKT GKPMINLYTDKDTGKPKGEA TVSFDDPPSAKAAIDWFDGK EFHGNIIKVSFATRRPE

Data sets:
Data typeCount
13C chemical shifts412
15N chemical shifts100
1H chemical shifts633

Additional metadata:

  • Assembly
  • Samples and Experiments
  • Software
  • Spectrometers
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Assembly:

Entity Assembly IDEntity NameEntity ID
1Taf151

Entities:

Entity 1, Taf15 97 residues - Formula weight is not available

1   GLYSERHISMETSERASPASNASNTHRILE
2   PHEVALGLNGLYLEUGLYGLUGLYVALSER
3   THRASPGLNVALGLYGLUPHEPHELYSGLN
4   ILEGLYILEILELYSTHRASNLYSLYSTHR
5   GLYLYSPROMETILEASNLEUTYRTHRASP
6   LYSASPTHRGLYLYSPROLYSGLYGLUALA
7   THRVALSERPHEASPASPPROPROSERALA
8   LYSALAALAILEASPTRPPHEASPGLYLYS
9   GLUPHEHISGLYASNILEILELYSVALSER
10   PHEALATHRARGARGPROGLU

Samples:

sample_1: Taf15, [U-100% 13C; U-100% 15N], 1 mM; H2O 90%; D2O 10%

sample_conditions_1: ionic strength: 0.05 M; pH: 6.2; pressure: 1 atm; temperature: 298 K

Experiments:

NameSampleSample stateSample conditions
2D 1H-15N HSQCsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aliphaticsample_1isotropicsample_conditions_1
2D 1H-13C HSQC aromaticsample_1isotropicsample_conditions_1
3D CBCA(CO)NHsample_1isotropicsample_conditions_1
3D HNCOsample_1isotropicsample_conditions_1
3D HNCAsample_1isotropicsample_conditions_1
3D HNCACBsample_1isotropicsample_conditions_1
3D HN(CO)CAsample_1isotropicsample_conditions_1
3D 1H-15N NOESYsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aliphaticsample_1isotropicsample_conditions_1
3D 1H-13C NOESY aromaticsample_1isotropicsample_conditions_1
3D H(CCO)NHsample_1isotropicsample_conditions_1
3D C(CO)NHsample_1isotropicsample_conditions_1

Software:

TOPSPIN v2.1, Bruker Biospin - processing

NMR spectrometers:

  • Bruker Avance 500 MHz
  • Bruker Avance 700 MHz

Related Database Links:

BMRB 19561
PDB
DBJ BAA33811 BAA33812 BAB46889 BAC34676 BAE27086
EMBL CAA67398
GB AAC50932 AAH46099 AAI37592 AAI66769 AAO13485
REF NP_001099294 NP_001302693 NP_003478 NP_081703 NP_631961
SP Q92804
TPG DAA19087
AlphaFold Q92804

Download HSQC peak lists in one of the following formats:
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SPARKY: Backbone or all simulated peaks