BMRB Entry 19561

Name: 1H, 13C and 15N backbone NMR assignments of human Taf15-RRM-RanBP
Published: 2014-04-11

Chem Shift validation: AVS_full, LACS
BMRB Entry DOI: doi:10.13018/BMR19561

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Nicholas Rego and David Koes
3Dmol.js: molecular visualization with WebGL
Bioinformatics (2015) 31 (8): 1322-1324 doi:10.1093/bioinformatics/btu829

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Title:

1H, 13C and 15N backbone NMR assignments of human Taf15-RRM-RanBP

Deposition date:

2013-10-16

Original release date:

2014-04-11

Authors:

Kashyap, Maruthi; Ganguly, Akshay Kumar; Bhavesh, Neel Sarovar

Citation:

Citation: Kashyap, Maruthi; Ganguly, Akshay Kumar; Bhavesh, Neel Sarovar. "Sequence-specific resonance assignments of human TAF15-RRM and TAF15-RRM-RanBP2." Biomol. NMR Assignments ., .-. (2014).
PubMed: 24659459

Assembly members:

Assembly members:
Taf-RRM-RanBP, polymer, 162 residues, Formula weight is not available

Natural source:

Natural source: Common Name: human Taxonomy ID: 9606 Superkingdom: Eukaryota Kingdom: Metazoa Genus/species: Homo sapiens

Experimental source:

Experimental source: Production method: recombinant technology Host organism: Escherichia coli Vector: pET 28a

Entity Sequences (FASTA):

Entity Sequences (FASTA):
Taf-RRM-RanBP: GSHMSDNNTIFVQGLGEGVS TDQVGEFFKQIGIIKTNKKT GKPMINLYTDKDTGKPKGEA TVSFDDPPSAKAAIDWFDGK EFHGNIIKVSFATRRPEFMR GGGSGGGRRGRGGYRGRGGF QGRGGDPKSGDWVCPNPSCG NMNFARRNSCNQCNEPRPED SR

Data sets:

assigned_chemical_shifts
- assigned_chem_shift_list_1

Data type	Count
13C chemical shifts	414
15N chemical shifts	146
1H chemical shifts	146

Additional metadata:

Assembly
Samples and Experiments
Software
Spectrometers
Hide all

Assembly:

Entity Assembly ID	Entity Name	Entity ID
1	Taf-RRM-RanBP	1

Entities:

Entity 1, Taf-RRM-RanBP 162 residues - Formula weight is not available

1

GLY

SER

HIS

MET

SER

ASP

ASN

THR

ILE

2

PHE

VAL

GLN

GLY

LEU

GLY

GLU

GLY

VAL

SER

3

THR

ASP

GLN

VAL

GLY

GLU

PHE

LYS

GLN

4

ILE

GLY

ILE

LYS

THR

ASN

LYS

THR

5

GLY

LYS

PRO

MET

ILE

ASN

LEU

TYR

THR

ASP

6

LYS

ASP

THR

GLY

LYS

PRO

LYS

GLY

GLU

ALA

7

THR

VAL

SER

PHE

ASP

PRO

SER

ALA

8

LYS

ALA

ILE

ASP

TRP

PHE

ASP

GLY

LYS

9

GLU

PHE

HIS

GLY

ASN

ILE

LYS

VAL

SER

10

PHE

ALA

THR

ARG

PRO

GLU

PHE

MET

ARG

11

GLY

SER

GLY

ARG

GLY

12

ARG

GLY

TYR

ARG

GLY

ARG

GLY

PHE

13

GLN

GLY

ARG

GLY

ASP

PRO

LYS

SER

GLY

14

ASP

TRP

VAL

CYS

PRO

ASN

PRO

SER

CYS

GLY

15

ASN

MET

ASN

PHE

ALA

ARG

ASN

SER

CYS

16

ASN

GLN

CYS

ASN

GLU

PRO

ARG

PRO

GLU

ASP

17

SER

ARG

Samples:

sample_1: Taf-RRM-RanBP, [U-13C; U-15N; U-2H], 1 mM; H2O 90%; D2O, [U-100% 2H], 10%; Na2SO4 50 mM; 2-mercaptoethanol 5 mM; phosphate buffer 20 mM

sample_conditions_1: ionic strength: 0.05 M; pH: 6; pressure: 1 atm; temperature: 298 K

Experiments:

Name	Sample	Sample state	Sample conditions
2D 1H-15N HSQC	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aliphatic	sample_1	isotropic	sample_conditions_1
2D 1H-13C HSQC aromatic	sample_1	isotropic	sample_conditions_1
3D HNCACB	sample_1	isotropic	sample_conditions_1
3D CBCA(CO)NH	sample_1	isotropic	sample_conditions_1
3D HN(CO)CA	sample_1	isotropic	sample_conditions_1
3D HNCO	sample_1	isotropic	sample_conditions_1
3D HNCA	sample_1	isotropic	sample_conditions_1
3D 1H-15N NOESY	sample_1	isotropic	sample_conditions_1

Software:

TOPSPIN, Bruker Biospin - collection, processing

NMR spectrometers:

Bruker Avance 700 MHz

BMRB	19320
PDB	2MMY
DBJ	BAA33811 BAA33812 BAB46889 BAC34676 BAE27086
EMBL	CAA67398
GB	AAC50932 AAH46099 AAI37592 AAI66769 AAO13485
REF	NP_001099294 NP_001302693 NP_003478 NP_081703 NP_631961
SP	Q92804
TPG	DAA19087
AlphaFold	Q92804